Anti-Profilin 1 antibody - Carboxyterminal end (ab50667)

  Immunoprecipitation - Profilin 1 antibody - Carboxyterminal end (ab50667)

Lane 1: Positve control — Western blot: ab50667 at a concentration of 1μg/ml on whole extract of Mouse 3T3 cells at 10μg/ml.

Lane 2: Immunoprecipitation — 10 μg of ab50667 was used to immunoprecipitate Mouse Profilin 1 from 3T3 cell lysate (20μg/ml). Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody.

Lane 3: Negative control — non-relevant antibody was used for IP. Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody

  Flow Cytometry - Profilin 1 antibody - Carboxyterminal end (ab50667)

ab50667 detecting Profilin 1 in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA and permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The cell population was gated on platelets. The primary antibody was diluted 1/200 in 2% BSA in PBS and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Chicken anti-Rabbit IgG (H+L) antibody, diluted 1/500 was used as the secondary antibody.

Abbreviations in the figure:
US Unstained, Red Peak;
IgG Rb: IgG Rabbit (Isotype Control), Blue Peak;
Profilin: Profilin 1, Green peak.

This image is courtesy of an Abreview by Mahesh Shivananjappa.

See Abreview

  Western blot - Anti-Profilin 1 antibody - Carboxyterminal end (ab50667)

Anti-Profilin 1 antibody - Carboxyterminal end (ab50667) at 1/1000 dilution + Human platelet lysate at 20 µg

Secondary
HRP-conjugated goat anti-rabbit polyclonal at 1/10000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)


Exposure time : 1 minute

Image courtesy of Mahesh Shivananjappa, United States

See Abreview