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Anti-Progesterone Receptor antibody [N559] (ab58565)

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Overview

  • Product nameAnti-Progesterone Receptor antibody [N559]See all Progesterone Receptor primary antibodies ...
  • Description
    Mouse monoclonal [N559] to Progesterone Receptor
  • SpecificityHuman progesterone receptor ( A and B isoforms)
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide to human progesterone receptor (PR). Amino-terminal domain aa. 551-564 (ASQSPQTSFESLPQ)

Properties

Applications

Our Abpromise guarantee covers the use of ab58565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution.
IP IP: Use at an assay dependent dilution.

Target

  • FunctionThe steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • Cellular localizationNucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases and Nucleus. Cytoplasm. Mainly nuclear.

    • Target information above from: UniProt accession P06401 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        NR3C3 antibodyNuclear receptor subfamily 3 group C member 3 antibodyPGR antibody
        PR antibodyPRA antibodyPRB antibodyPRGR_HUMAN antibodyProgesterone receptor antibodyProgestin receptor form A antibodyProgestin receptor form B antibody
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    References for Anti-Progesterone Receptor antibody [N559] (ab58565)

    ab58565 has not yet been referenced specifically in any publications.

    Publishing research using ab58565 ? Please let us know so that we can cite the reference in this datasheet

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