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Anti-Progesterone Receptor antibody [Y85] (ab32085)

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Overview

Product name

Anti-Progesterone Receptor antibody [Y85]
See all Progesterone Receptor products (37) ...

Description

Rabbit monoclonal [Y85] to Progesterone Receptor

Specificity

ab32085 recognises progesterone receptor. The antibody does not cross-react with other NR3 family members. Since the recognized epitope is near the N-terminal end of the protein, this product should only detect isoform B and not isoform A.

Tested applications

WB, IHC-P, ICC, IPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to residues near N terminus of human Progesterone Receptor

Positive control

Human breast carcinoma, T47D cell lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Y85

Isotype

IgG

Research areas

Cancer >> Tumor biomarkers >> Receptors
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Progesterone
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Progesterone
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Progesterone
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

  • Western blot - Progesterone Receptor antibody [Y85] (ab32085)Western blot - Progesterone Receptor antibody [Y85] (ab32085) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Progesterone Receptor antibody [Y85] (ab32085)Immunohistochemistry (Paraffin-embedded sections) - Progesterone Receptor antibody [Y85] (ab32085) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/10000. Predicted m...Read more →

    WB: 1/10000. Predicted molecular weight: 99 kDa.

  • 3 Images

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • ICC

     ICC: 1/50.

    ICC: 1/50.

  • IP

     IP: 1/150.

    IP: 1/150.

Application notes

Is unsuitable for or Flow Cyt.

Target

Function

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Post-translational
modifications

Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.

Cellular localization

Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases and Nucleus. Cytoplasm. Mainly nuclear.

Target information above from: UniProt accessionP06401 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • NR3C3 antibody
  • NR3C3 antibody
  • Nuclear receptor subfamily 3 group C member 3 antibody
  • PGR antibody
  • PGR antibody
  • PR antibody
  • PR antibody
  • PRA antibody
  • PRB antibody
  • PRGR_HUMAN antibody
  • Progesterone receptor antibody
  • Progestin receptor form A antibody
  • Progestin receptor form B antibody
see all

Database links

Anti-Progesterone Receptor antibody [Y85] images:

  Western blot - Progesterone Receptor antibody [Y85] (ab32085)

Western blot - Progesterone Receptor antibody [Y85] (ab32085)

Anti-Progesterone Receptor antibody [Y85] (ab32085) at 1/10000 dilution + T47D cell lysate

Predicted band size : 99 kDa
Observed band size : 99,135 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - Progesterone Receptor antibody [Y85] (ab32085)

Immunohistochemistry (Paraffin-embedded sections) - Progesterone Receptor antibody [Y85] (ab32085)

Immunohistochemical analysis of progesterone receptor expression in paraffin embedded human breast carcinoma, using 1/100 ab32085.

References for Anti-Progesterone Receptor antibody [Y85] (ab32085)

This product has been referenced in:

  • Yin P  et al. Progesterone receptor regulates Bcl-2 gene expression through direct binding to its promoter region in uterine leiomyoma cells. J Clin Endocrinol Metab 92:4459-66 (2007).Read more (PubMed: 17785366) »

See 1 publication for this product

Publishing research using ab32085? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"