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- Secondary antibodies
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Product name
Anti-Progesterone Receptor (phospho S294) antibody
See all Progesterone Receptor products (37) ...
Description
Rabbit polyclonal to Progesterone Receptor (phospho S294)
Specificity
The antibody is specific for human progesterone receptor phosphorylated at Ser294.
Tested applications
Dot Blot, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer294 of human progesterone receptor
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 150mM Sodium Chloride, 10mM HEPES, 100µg/ml BSA
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
The antibody was purified by ammonium sulphate followed by Protein G affinity purification methods.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Progesterone
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Progesterone
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Progesterone
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Western blot - Progesterone Receptor (phospho S294) antibody (ab13878)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot Blot
Dot: 1/1000 - 1/2000.Dot: 1/1000 - 1/2000.
WB: 1/1000 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
Target
Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Cellular localization
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases and Nucleus. Cytoplasm. Mainly nuclear.
Target information above from: UniProt accessionP06401
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NR3C3 antibody
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
see all
Database links
- Entrez Gene: 5241 Human
- Omim: 607311 Human
- SwissProt: P06401 Human
- Unigene: 2905 Human
- Unigene: 32405 Human
Anti-Progesterone Receptor (phospho S294) antibody images:
Western blot - Progesterone Receptor (phospho S294) antibody (ab13878)
Predicted band size : 99 kDa
Western blot analysis of progesterone receptor using ab13878.
References for Anti-Progesterone Receptor (phospho S294) antibody (ab13878)
ab13878 has not yet been referenced specifically in any publications.
Publishing research using ab13878? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"