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Anti-Progesterone Receptor (unmodified S190) antibody (ab39423)

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Overview

Product name

Anti-Progesterone Receptor (unmodified S190) antibody
See all Progesterone Receptor products (37) ...

Description

Rabbit polyclonal to Progesterone Receptor (unmodified S190)

Specificity

Ab39423 detects endogenous levels of total Progesterone Receptor protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide derived from Progesterone Receptor around the phosphorylation site of serine 190 (Human)

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Ab39423 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Tumor biomarkers >> Receptors
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Progesterone
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Progesterone
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Progesterone
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Applications

Show applications key

Our Abpromise guarantee covers the use of ab39423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    WB

     WB: 1/500 - 1/1000. Pred...Read more →

    WB: 1/500 - 1/1000. Predicted molecular weight: 99 kDa.

  • ELISA

     ELISA: 1/1000.

    ELISA: 1/1000.

Target

Function

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Post-translational
modifications

Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.

Cellular localization

Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases and Nucleus. Cytoplasm. Mainly nuclear.

Target information above from: UniProt accessionP06401 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • NR3C3 antibody
  • NR3C3 antibody
  • Nuclear receptor subfamily 3 group C member 3 antibody
  • PGR antibody
  • PGR antibody
  • PR antibody
  • PR antibody
  • PRA antibody
  • PRB antibody
  • PRGR_HUMAN antibody
  • Progesterone receptor antibody
  • Progestin receptor form A antibody
  • Progestin receptor form B antibody
see all

Database links

References for Anti-Progesterone Receptor (unmodified S190) antibody (ab39423)

ab39423 has not yet been referenced specifically in any publications.

Publishing research using ab39423? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"