Proprotein Convertase 2 antibody (ab3533)

Product Name 

Proprotein Convertase 2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Proprotein Convertase 2

Immunogen 

Synthetic peptide: ELDEAVERSLQSILRKN, corresponding to amino acids 622-638 of Mouse Proprotein Convertase 2(Peptide available as ab5012.).
BLAST 'ELDEAVERSLQSILRKN' with or

Reacts with 

Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Western Blot: use at a concentration of 2 µg/ml.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract.

Cellular localization 

Secretory vesicle. Localized in the secretion granules.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Signal Transduction >> Growth Factors/Hormones >> Hormones
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like

Relevance 

PC2 was initially found as an insulin processing enzyme, and was the third of the human prohormone convertasae enzymes to be discovered. Like furin, PC1 and PACE4, PC2 is a serine proteinase that cleaves after pairs of basic amino acids, but with some differences in substrate specificity and distribution. The catalytic domain has homology to other PC enzymes, the prohormone convertase family initially discovered for their role in processing POMC, insulin and other pro-hormones, and later found to process a wider range of precursor proteins. Some have renamed the prohormone convertases as proprotein convertases, and another group has renamed the entire family with a uniform nomenclature of SPCs ( s ubtilisin-like p roprotein c onvertases), with PC2 getting the SPC2 moniker. PC2 structure contains a propeptide domain, a catalytic domain and an RGD containing cystein-rich domain (homo-B). The PCs have an RxxR consensus cleavage requirement, and the propeptide is separated from the mature protein by just such a sequence. After cleavage of the propeptide domain, PC2 becomes a two-chain form, and the propeptide piece is released after a second internal cleavage. PC2 resides along with PC1, mainly in the dense core secretory vesicles and the TGN, and is cycled to the surface, with some of the PC2 being secreted from the cells. PC2 has been reported to be elevated in tumor cell lines and to increase tumor progression. PC2 is expressed at low levels in most tissues, but found in highest concentration in the brain, the anterior pituitary, pancreatic islet cells, thyroid, parathyroid and gut. PC2 cleaves a wide variety of proteins, including growth factors, viral coat proteins, matrix metalloproteinases, mostly after PC1 cleavage. The maturation of PC2 appears to require association with the 7B2 protein, which both helps fold PC2 properly and keeps the PC2 latent until the 7B2 protein is clipped by a PC enzyme.

The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS, 1 mg/ml BSA, 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Proprotein Convertase 2 antibody (ab3533)

  This product has been used in:

Gagnon J et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). PubMed: 18706454

If you publish research using ab3533 please let us know so that we can cite the reference on this datasheet.

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