Anti-Prokineticin 2/PK2 antibody (ab87360)
Key features and details
- Rabbit polyclonal to Prokineticin 2/PK2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Prokineticin 2/PK2 antibody
See all Prokineticin 2/PK2 primary antibodies -
Description
Rabbit polyclonal to Prokineticin 2/PK2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Macaque monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human Prokineticin 2/PK2 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab123828) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Testis; Human Small Intestine; Mouse Testis; Rat Testis.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 14 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 14 kDa). |
Target
-
Function
May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. -
Tissue specificity
Expressed in the testis and, at low levels, in the small intestine. -
Involvement in disease
Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4) [MIM:610628]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. -
Sequence similarities
Belongs to the AVIT (prokineticin) family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 101143069 Gorilla
- Entrez Gene: 60675 Human
- Entrez Gene: 50501 Mouse
- Entrez Gene: 192206 Rat
- Omim: 607002 Human
- SwissProt: Q9HC23 Human
- SwissProt: Q9QXU7 Mouse
- SwissProt: Q8R413 Rat
see all -
Alternative names
- BV8 antibody
- Bv8 homolog antibody
- MIT1 antibody
see all
Images
-
All lanes : Anti-Prokineticin 2/PK2 antibody (ab87360) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Testis (Mouse) Tissue Lysate
Lane 4 : Testis (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab87360 has been referenced in 3 publications.
- Tu Q et al. Prokineticin 2 promotes macrophages-mediated antibacterial host defense against bacterial pneumonia. Int J Infect Dis 125:103-113 (2022). PubMed: 36241161
- Zhang Y et al. MicroRNA-374a Governs Aggressive Cell Behaviors of Glioma by Targeting Prokineticin 2. Technol Cancer Res Treat 18:1533033818821401 (2019). PubMed: 30803356
- Maftei D et al. The prokineticin receptor antagonist PC1 rescues memory impairment induced by ß amyloid administration through the modulation of prokineticin system. Neuropharmacology 158:107739 (2019). PubMed: 31408628