![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prolactin Receptor antibody [B6.2] - BSA and Azide free (ab80565) image](#Prolactin-Primary-antibodies-ab80565-1.JPG)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-Prolactin Receptor antibody [B6.2] - BSA and Azide free
See all Prolactin Receptor products (10) ...
Description
Mouse monoclonal [B6.2] to Prolactin Receptor - BSA and Azide free
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Semi-purified human Prolactin Receptor.
Positive control
Breast carcinoma
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified from ascites fluid.
Clonality
Monoclonal
Clone number
B6.2
Isotype
IgG1
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prolactin Receptor antibody [B6.2] - BSA and Azide free (ab80565)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prolactin Receptor antibody [B6.2] - BSA and Azide free (ab80565) image](/ps/datasheet/images/80/ab80565/Prolactin-Primary-antibodies-ab80565-1.JPG)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab80565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: Use at a concentration of 1 - 2 µg/ml (for 30 minutes at room temperature). Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1 mg/ml (in Tris-HCl, pH 2.0 for 15 minutes at room temperature or 10 minutes at 37 °C).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
Tissue specificity
Expressed in breast, placenta, kidney, liver and pancreas.
Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains.
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Cellular localization
Secreted and Membrane.
Target information above from: UniProt accessionP16471
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI987712 antibody
- CLONE SPM213 antibody
- CPRLP antibody
see all
Database links
- Entrez Gene: 5618 Human
- Omim: 176761 Human
- SwissProt: P16471 Human
- SwissProt: Q96P35 Human
- SwissProt: Q96P36 Human
- Unigene: 368587 Human
Anti-Prolactin Receptor antibody [B6.2] - BSA and Azide free images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prolactin Receptor antibody [B6.2] - BSA and Azide free (ab80565)
Formalin fixed, paraffin embedded human breast cancer stained with ab80565 at 2 µg/ml. Peroxidase conjugate and AEC chromogen were used. Note cell membrane and cytoplasmic staining of tumor cells.
References for Anti-Prolactin Receptor antibody [B6.2] - BSA and Azide free (ab80565)
ab80565 has not yet been referenced specifically in any publications.
Publishing research using ab80565? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"