![- Prolactin Receptor antibody [T6] (ab2773) image](#ab2733_1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-Prolactin Receptor antibody [T6]
See all Prolactin Receptor products (10) ...
Description
Mouse monoclonal [T6] to Prolactin Receptor
Specificity
Detects prolactin (PRL) receptor in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing PRL receptor in NB2 cell lysate. Immunohistochemical staining of PRL receptor in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining. This antibody has also been used to inhibit the binding of prolactin to prolactin receptor in vitro.
Tested applications
ICC, IHC-P, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Rat
Immunogen
Purified rat liver PRL receptor.
Positive control
WB and ICC: NB2 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
Clonality
Monoclonal
Clone number
T6
Isotype
IgG2a
-
- Prolactin Receptor antibody [T6] (ab2773)
![- Prolactin Receptor antibody [T6] (ab2773) image](/ps/datasheet/Images/2/ab2773/ab2733_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 5 µg/ml
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 42 kDa.
Target
Function
This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
Tissue specificity
Expressed in breast, placenta, kidney, liver and pancreas.
Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains.
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Cellular localization
Secreted and Membrane.
Target information above from: UniProt accessionP16471
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI987712 antibody
- CLONE SPM213 antibody
- CPRLP antibody
see all
Database links
Anti-Prolactin Receptor antibody [T6] images:
- Prolactin Receptor antibody [T6] (ab2773)
Immunohistochemical staining of PRL receptor in NB2 cells using ab2733.
References for Anti-Prolactin Receptor antibody [T6] (ab2773)
This product has been referenced in:
- Dardenne Met al. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endocrinology 134:2108-14 (1994).Read more (PubMed: 8156910) »
- Rui Het al. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology 135:1299-306 (1994). Read more (PubMed: 7925093) »
See all 6 publications for this product
Publishing research using ab2773? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"