Prolactin Receptor antibody [T6] (ab2773)

Product Name 

Prolactin Receptor antibody [T6]

Product type 

Primary antibodies

Description 

Mouse monoclonal [T6] to Prolactin Receptor

Immunogen 

Purified rat liver PRL receptor.

Reacts with 
(species key)

Rat

Specificity 

Detects prolactin (PRL) receptor in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing PRL receptor in NB2 cell lysate. Immunohistochemical staining of PRL receptor in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining.
This antibody has also been used to inhibit the binding of prolactin to prolactin receptor in vitro.

Tested applications 
(see key)

ICC, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: Use at a concentration of 5 µg/ml.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 42 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB and ICC: NB2 cells

Cellular localization 

Plasma Membrane; Single-pass type I membrane protein. Secreted

Research areas 

Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

Relevance 

Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription. Three isoforms of Prolactin receptor have been identified.

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

T6

Isotype 

IgG2a

Purity 

IgG fraction

Storage buffer 

n/a

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Prolactin Receptor antibody [T6] (ab2773)

  This product has been used in: (two most recent references)

Dardenne M et al. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endocrinology 134:2108-14 (1994). PubMed: 8156910

Rui H et al. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology 135:1299-306 (1994). PubMed: 7925093

See all 6 publication references for this product.


If you publish research using ab2773 please let us know so that we can cite the reference on this datasheet.

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