Prolactin Receptor antibody [U5] (ab2772)

Product Name 

Prolactin Receptor antibody [U5]

Product type 

Primary antibodies

Description 

Mouse monoclonal [U5] to Prolactin Receptor

Immunogen 

Purified rat liver PRL receptor.

Reacts with 
(species key)

Hu, Rat, Pig, Rb

Specificity 

Detects prolactin (PRL) receptor.

Epitope 

This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Immunocytochemistry 5 µg/ml
Western Blot 1 µg/ml
By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues.
Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with ab2772 results in a staining pattern consistent with cytoplasmic vesicle staining.
ab2772, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS.
ab2772 does not inhibit the interaction of prolactin with the receptor.

Cellular localization 

Plasma Membrane; Single-pass type I membrane protein. Secreted

Research areas 

Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

Relevance 

Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription. Three isoforms of Prolactin receptor have been identified.

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

U5

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Constituents: PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Prolactin Receptor antibody [U5] (ab2772)

  This product has been used in: (two most recent references)

Perrot-Applanat M et al. Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation. J Cell Sci 110 (Pt 9):1123-32 (1997). PubMed: 9175708

Royster M et al. The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation. Endocrinology 136:3892-900 (1995). PubMed: 7649097

See all 11 publication references for this product.


If you publish research using ab2772 please let us know so that we can cite the reference on this datasheet.

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