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Product name
Anti-Prolyl Endopeptidase antibody
See all Prolyl Endopeptidase products (7) ...
Description
Rabbit polyclonal to Prolyl Endopeptidase
Specificity
ab58988 does not recognize other Prolyl Endopeptidase family members.
Tested applications
WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human
Immunogen
Synthetic peptide based on the amino terminal end of human Prolyl Endopeptidase. (Peptide available as ab639 73.) Immunizing peptide available on request, please contact a customer service representative to ask for availability.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
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Western blot - Prolyl Endopeptidase antibody (ab58988)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 - 1/5000 dependent on substrate. Predicted molecular weight: 81 kDa.
A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.
EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
Sequence similarities
Belongs to the peptidase S9A family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP48147
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- dJ355L5.1 (prolyl endopeptidase) antibody
- HGNC:9358 antibody
- MGC16060 antibody
see all
Database links
- Entrez Gene: 5550 Human
- Entrez Gene: 19072 Mouse
- Entrez Gene: 83471 Rat
- Omim: 600400 Human
- SwissProt: Q8N6D4 Human
- SwissProt: P48147 Human
- SwissProt: Q9QUR6 Mouse
- SwissProt: O70196 Rat
see all
Anti-Prolyl Endopeptidase antibody images:
Western blot - Prolyl Endopeptidase antibody (ab58988)
All lanes : Anti-Prolyl Endopeptidase antibody (ab58988) at 1/1000 dilution
Lane 1 : Madin Darby canine kidney (MDCK) cell lysate
at 10 µl
Lane 2 : Madin Darby canine kidney (MDCK) cell lysate
at 5 µl
Predicted band size : 81 kDa
References for Anti-Prolyl Endopeptidase antibody (ab58988)
This product has been referenced in:
- Perroud B et al. In vivo multiplex quantitative analysis of 3 forms of alpha melanocyte stimulating hormone in pituitary of prolyl endopeptidase deficient mice. Mol Brain 2:14 (2009). WB; Mouse.Read more (PubMed: 19490636) »
- Warden CH et al. Maternal influence of prolyl endopeptidase on fat mass of adult progeny. Int J Obes (Lond) 33:1013-22 (2009). WB; Mouse.Read more (PubMed: 19564876) »
See all 2 publications for this product
Publishing research using ab58988? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"