Propidium Iodide (ab14083)
- Product namePropidium Iodide
- Product overview
Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.
For Microscopy analysis: PI can be viewed using rhodamine(red) filter (λ= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).
Flow Cytometry analysis: PI staining can be monitored in FL2 (DNA content) or FL3 (viability) channel.
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- Tested applicationsFluorescence Microscopy more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 ml Propidium Iodide (1mg/ml) ab14083 1 x 1ml
- RelevancePropidium Iodide (PI) (MW=668.4 Da) is an intercalating agent and a fluorescent molecule which is membrane impermeant and generally excluded from viable cells. Upon entering cells, PI will bind to DNA and RNA by intercalating between bases. Once bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Exmax= 536nm / Emmax= 617 nm.
Our Abpromise guarantee covers the use of ab14083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Fluorescence Microscopy||Fluorescence Microscopy|
References for Propidium Iodide (ab14083)
This product has been referenced in:
- Li M et al. High FSH decreases the developmental potential of mouse oocytes and resulting fertilized embryos, but does not influence offspring physiology and behavior in vitro or in vivo. Hum Reprod 28:1309-23 (2013). Read more (PubMed: 23411618) »
- Riedl S et al. In search of a novel target - phosphatidylserine exposed by non-apoptotic tumor cells and metastases of malignancies with poor treatment efficacy. Biochim Biophys Acta 1808:2638-45 (2011). Read more (PubMed: 21810406) »