Overview
Fluorescent β2 adrenoceptor antagonist
β2-633-AN
Excitation wavelength: 633 nm
Emission wavelength: 650 nm
Properties
To determine the KD for ab118168 at β1, β2 and β3 receptors, cells were treated with concentrations of isoprenaline alone, or in the presence of 1 µM ab118168, and the cyclic AMP-induced expression of SPAP measured.
The KD was calculated from the shift of the agonist response curve in the presence of ab118168, compared to the response curve for the agonist alone, for β1, β2 and β3 receptor expressing cell lines.
A β2 adrenoceptor CHO-K1 cell line was also treated as above.
Applications
Our Abpromise guarantee covers the use of ab118168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Small Molecule Fluorescent Optical Detection | Small Molecule Fluorescent Optical Detection: Use at an assay dependent concentration. |
| Receptor Localization | Receptor Localization: Use at an assay dependent concentration. |
| Fluorescent Cell Imaging | Fluorescent Cell Imaging: Use at an assay dependent concentration. |
Propranolol Fluorescent ligand (Red) images
-
Receptor Localization - Propranolol Fluorescent ligand (Red) (ab118168)ab118168 binding and displacement. Top (A, C, E): ab118168 ligand (A: 30 nM; C, E: 100 nM) binding to three different live cell lines expressing β2 adrenergic receptors. Bottom (B, D, F): Binding blocked by the unlabelled competitor ICI 118551 (10 µM). Nuclei have been counter-stained with Hoechst. -
Small Molecule Fluorescent Optical Detection - Propranolol Fluorescent ligand (Red) (ab118168)To determine the apparent KD for ab118168 at β1 receptors, cells were treated with varying concentrations of isoprenaline alone, or in the presence of ab118168, and the cyclic AMP-induced expression of SPAP measured. The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of ab118168, compared to the response curve for the agonist alone, for a β1 receptor expressing cell line. -
Small Molecule Fluorescent Optical Detection - Propranolol Fluorescent ligand (Red) (ab118168)To determine the apparent KD for ab118168 at β2 receptors, cells were treated with varying concentrations of isoprenaline alone, or in the presence of ab118168, and the cyclic AMP-induced expression of SPAP measured. The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of ab118168, compared to the response curve for the agonist alone, for a β2 receptor expressing cell line. -
Small Molecule Fluorescent Optical Detection - Propranolol Fluorescent ligand (Red) (ab118168)A β2 adrenoceptor CHO-K1 cell line was also transfected with the cAMP-responsive SPAP reporter construct and tested for its functional response to varying concentrations of isoprenaline alone, or in the presence of 1 µM ab118168.
References for Propranolol Fluorescent ligand (Red) (ab118168)
ab118168 has not yet been referenced specifically in any publications.
