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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Overview
Product name
Anti-Proprotein convertase PC4 antibody - Aminoterminal end
See all Proprotein convertase PC4 products (2) ...
Description
Rabbit polyclonal to Proprotein convertase PC4 - Aminoterminal end
Specificity
ab28573 recognises the Amino end of mature enzyme PC4.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the the amino end of mature human PC4.
(Peptide available as ab41410.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
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Immunocytochemistry/ Immunofluorescence-Proprotein convertase PC4 antibody - Aminoterminal end(ab28573)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
WB
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 83 kDa but shed PC4 is seen as a 54 kDa band on reduced Western blots with smaller bands seen as the PC4 is further processed. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
PC4 was the fifth of the human prohormone convertasae enzymes to be discovered. Like furin, PC1, PC2 and PACE4, PC4 is a serine proteinase that cleaves after pairs of basic amino acids, but with some differences in substrate specificity and distribution. The catalytic domain has homology to other PC enzymes, the prohormone convertase family initially discovered for their role in processing POMC, insulin and other pro-hormones, and later found to process a wider range of precursor proteins. Some have renamed the prohormone convertases as proprotein convertases, and another group has renamed the entire family with a uniform nomenclature of SPCs ( s ubtilisin-like p roprotein c onvertases). PC4 structure contains a propeptide domain, a catalytic domain and an RGD containing cystein-rich domain (homo-B). The PCs have an RxxR consensus cleavage requirement, and the propeptide is separated from the mature protein by just such a sequence. After cleavage of the propeptide domain, PC4 becomes a two-chain form, and the propeptide piece is released after a second internal cleavage.
Cellular localization
Membrane; single pass membrane protein
Alternative names
- PC4 antibody
- PCSK4 antibody
- Proprotein convertase PC4 antibody
- Proprotein convertase subtilisin/kexin type 4 antibody
Database links
- Entrez Gene: 54760 Human
- Omim: 600487 Human
- SwissProt: Q6UW60 Human
- Unigene: 46884 Human
Anti-Proprotein convertase PC4 antibody - Aminoterminal end images:
Immunocytochemistry/ Immunofluorescence-Proprotein convertase PC4 antibody - Aminoterminal end(ab28573)
ICC/IF image of ab28573 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28573, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Proprotein convertase PC4 antibody - Aminoterminal end (ab28573)
ab28573 has not yet been referenced specifically in any publications.
Publishing research using ab28573? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"