Proprotein convertase PC4 peptide (Aminoterminal end) (ab41410)

Product Name 

Proprotein convertase PC4 peptide (Aminoterminal end)

Product type 

Proteins and Peptides

Peptide 

Synthetic peptide PC4.


This peptide was used as an immunogen for ab28573 - PC4 antibody - Aminoterminal end.

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all Proprotein convertase PC4 antibodies

Tested applications 
(see key)

ELISA, WB

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab28573. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases

Relevance 

PC4 was the fifth of the human prohormone convertasae enzymes to be discovered. Like furin, PC1, PC2 and PACE4, PC4 is a serine proteinase that cleaves after pairs of basic amino acids, but with some differences in substrate specificity and distribution. The catalytic domain has homology to other PC enzymes, the prohormone convertase family initially discovered for their role in processing POMC, insulin and other pro-hormones, and later found to process a wider range of precursor proteins. Some have renamed the prohormone convertases as proprotein convertases, and another group has renamed the entire family with a uniform nomenclature of SPCs ( s ubtilisin-like p roprotein c onvertases). PC4 structure contains a propeptide domain, a catalytic domain and an RGD containing cystein-rich domain (homo-B). The PCs have an RxxR consensus cleavage requirement, and the propeptide is separated from the mature protein by just such a sequence. After cleavage of the propeptide domain, PC4 becomes a two-chain form, and the propeptide piece is released after a second internal cleavage.

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Customer reviews (feedback) regarding Proprotein convertase PC4 peptide (Aminoterminal end)

Customer FAQs regarding Proprotein convertase PC4 peptide (Aminoterminal end)

Protocols for Proprotein convertase PC4 peptide (Aminoterminal end)

Price and availability of products related to Proprotein convertase PC4 peptide (Aminoterminal end)

Proprotein convertase PC4 peptide (Aminoterminal end) - related products: