Overview

  • Product name
    Anti-Prostaglandin D2 Receptor antibody
    See all Prostaglandin D2 Receptor primary antibodies
  • Description
    Rabbit polyclonal to Prostaglandin D2 Receptor
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse Prostaglandin D2 Receptor aa 300 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P70263
    (Peptide available as ab111326)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: NIH3T3; EL4; Raw 264.7; A549; HepG2 and in the following tissue lysates: Mouse lung; Mouse brain; Mouse kidney; Rat small intestine; Rat kidney.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Target

  • Function
    Receptor for prostaglandin D2 (PGD2). The activity of this receptor is mainly mediated by G(s) proteins that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. A mobilization of calcium is also observed, but without formation of inositol 1,4,5-trisphosphate.
  • Tissue specificity
    Expressed in retinal choroid, ciliary epithelium, longitudinal and circular ciliary muscles, iris, small intestine and platelet membranes.
  • Involvement in disease
    Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1) [MIM:607277]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AS 1 antibody
    • AS1 antibody
    • ASRT 1 antibody
    • ASRT1 antibody
    • DP antibody
    • MGC49004 antibody
    • OTTHUMP00000178986 antibody
    • PD2R_HUMAN antibody
    • PGD receptor antibody
    • PGD2 receptor antibody
    • Prostaglandin D2 receptor (DP) antibody
    • Prostaglandin D2 receptor antibody
    • Prostanoid DP receptor antibody
    • Ptgdr antibody
    see all

Images

  • All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml

    Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : EL4 whole cell lysate (ab7183)
    Lane 6 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 87 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Small Intestine (Rat) Tissue Lysate
    Lane 4 : Kidney (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References

This product has been referenced in:
  • Guan NN  et al. Prostaglandin D2 effects and DP1 /DP2 receptor distribution in guinea pig urinary bladder out-flow region. J Cell Mol Med 21:234-243 (2017). IHC . Read more (PubMed: 27664012) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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