Anti-Prostaglandin E Receptor EP4 antibody (ab45863)

Overview

  • Product name
    Anti-Prostaglandin E Receptor EP4 antibody
    See all Prostaglandin E Receptor EP4 primary antibodies
  • Description
    Goat polyclonal to Prostaglandin E Receptor EP4
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Dog, Chimpanzee, Baboon, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    C-LEREVSKNPDLQA

    , corresponding to Internal sequence amino acids 302-314 of Human Prostaglandin E Receptor EP4

  • Positive control
    • Human Testes lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
    • Tissue specificity
      High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EP 4 antibody
      • EP4 antibody
      • EP4R antibody
      • MGC126583 antibody
      • PE2R4_HUMAN antibody
      • PGE receptor EP4 subtype antibody
      • PGE2 receptor EP4 subtype antibody
      • Prostaglandin E receptor 4 antibody
      • Prostaglandin E receptor 4 subtype EP4 antibody
      • Prostaglandin E2 receptor antibody
      • Prostaglandin E2 receptor EP4 subtype antibody
      • Prostanoid EP4 receptor antibody
      • PTGER 2 antibody
      • PTGER 4 antibody
      • PTGER2 antibody
      • PTGER4 antibody
      see all

    Images

    • Anti-Prostaglandin E Receptor EP4 antibody (ab45863) at 0.1 µg/ml + Human Testes lysate (35µg protein in RIPA buffer)

      Predicted band size : 53 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    References

    ab45863 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab45863.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up