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Prostate Specific Antigen Human ELISA Kit (ab113327)

Submit an Abreview Q&A (8)

Overview

  • Product nameProstate Specific Antigen Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 8 pg/ml
  • Range
    10.24 pg/ml - 2500 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 81% - 108%
    Serum 105.4 94% - 112%
    Plasma 80.63 72% - 94%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab113327 Prostate Specific Antigen (PSA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PSA in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human PSA coated on a 96-well plate. Standards and samples are pipetted into the wells and PSA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PSA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PSA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P07288 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        APSGamma seminoproteinGamma-seminoprotein
        Gamma-seminoproteinhK3Kallikrein 3Kallikrein related peptidase 3Kallikrein-3Kallikrein-3KLK 3KLK2A1KLK3Klk3KLK3_HUMANKLK3_HUMANP 30 antigenP-30 antigenP-30 antigenP30 antigenProstate Specific AntigenProstate-specific antigenProstate-specific antigenPsaPsaSemenogelaseSemenogelaseSemininSeminin
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab113327 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Prostate Specific Antigen Human ELISA Kit images

      • ELISA - PSA Human ELISA Kit (ab113327)
        ELISA - PSA Human ELISA Kit (ab113327)

      Protocols

      References for Prostate Specific Antigen Human ELISA Kit (ab113327)

      ab113327 has not yet been referenced specifically in any publications.

      Publishing research using ab113327 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab113327 Submit a Question for ab113327

      Displaying 1 - 8 of 8 results for Abreviews and Q&A

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      I had a quick question regarding the Prostate Specific Antigen Human ELISA kit (ab113327). We will be determing the PSA levels in human plasma. I noticed in the protocol guide that Assay Diluent A should be used for these samples. However, the Detection A...

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      Assay diluent A is is only required for the preparation of the standards and samples, if your samples are plasma. Once the PSA is captured on the plate and the samples and standard solutions are discarded from the wells, the remainder of the assay does no...

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      Phoned because the blank sample/standard are very high.

      Thank you for contacting us. Your credit note ID is . I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing depar...

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      thank you for your offer. I would like to try one more time and see if I can get some reading for our samples. So I would like to get a replacement kit free of charge. The PO# of the original kit was:  

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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      I am attaching the data from the abcam human PSA ELISA kit. We were just trying out the kit and didn't run the standard in duplicate. In the first data set we ran the standards and one lab pooled human serum (reading 0.7) and two test samples (CT1L1 and C...

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      Thank you for your message and for providing this further information.

      I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to offer ...

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      May be you didn't understand the problem. There is no problem with the samples. It is the blank and the lower concentrations of the standard that give high readings. The sample reading is fine. I specially selected the abcam ELISA kit because it is ad...

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      Thank you for your response.

      I am sorry that the initial suggestion that I was able to give and that these further suggestions did not improve blank reading and thereby the detection limits of this for you. Would you be willing to send me the d...

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      Phoned because the blank sample/standard are very high.

      Thank you for contacting Abcam regarding your higher 0 reading.

      I have had a chance to speak with the lab regarding this. Their reply follows:

      In general, a 0 standard OD reading around 0.2 OD is not considered very high but is in th...

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      Bonjour

      Merci de votre réponse rapide

      Le kit ELISA que nous souhaterions commander est celui-ci:

      http://www.abcam.com/prostate-specific-antigen-human-elisa-kit-ab113327.html

      Bien cordialement

      Code de remise : XX
      Date d’expiration : 09. Juillet 2012

      Je suis ravie que vous souhaitiez participer a notre offre et tester ab113327 chez le macaque. Le code de réduction correspondant est XX. Ce code de réduction est utilisable sous 120...

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       Dear Tech Support Team,   From your experience, kindly recommend the dilutions of urine that the customer should perform with ab113327.   Thanks in advance for your assistance and reply.   Best Regards,      

      Thank you for your inquiry. Although our production team does not’t routinely test urine samples,we know this kit is suitable for urine samples (based on our past experience with it). This is a general guideline on preparation of urine for ELISA t...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"