Anti-Prostate Specific Antigen antibody [2H9] (ab10184)

Overview

  • Product nameAnti-Prostate Specific Antigen antibody [2H9]See all Prostate Specific Antigen primary antibodies ...
  • Description
    Mouse monoclonal [2H9] to Prostate Specific Antigen
  • SpecificityEquimolar total PSA, epitope 3, 50% cross reactivity with human kallikrein 2.
  • Tested applicationsSandwich ELISA, ICC/IF, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human PSA antigen purified from semenal plasma.

  • Positive control
    • IF/ICC: PC3 cell line.
  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab10184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187) and Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466).

Detection of PSA antigen. Recommended pairs for detection of total PSA (capture - detection):

ab10184 (capture) - ab10185 (detection)

ab24466 (capture) - ab10184 (detection)

Recommended pairs for detection of free PSA (capture - detection):

ab10184 (capture) - ab10187 (detection)

ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P07288 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • KLK3 antibody
    • KLK3_HUMAN antibody
    • P 30 antigen antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate Specific Antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

Anti-Prostate Specific Antigen antibody [2H9] images

  • ICC/IF image of ab10184 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10184, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Prostate Specific Antigen antibody [2H9] (ab10184)

ab10184 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. None of these products contain BSA. We do have the storage buffer listed on the datasheets for your convenience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ...

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Thank you very much for your call yesterday and for your patience while I've been in touch with the lab.

These antibodies were tested together in sELISA against purified native PSA, not with serum samples.

I hope this information wi...

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Thank you for your enquiry.

We have used our internal sandwich ELISA protocol only to determine which monoclonal antibodies are working together as pairs. Usually any general protocol, like for example the one youfind below, with proper opti...

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Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab77310 has never been tested in sandwich ELISA (sELISA). It has been tested in indirect ELISA only (ELISA). All tested and guaranteed applications are specified ...

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Thank you for your reply.

I have reviewed the details of this experiment and have the following feedback:

1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab1028...

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Thank you for your enquiry.

Sandwich ELISA (sELISA) procedures can be difficult to optimize and tested match pair antibodies should be used. Any tested matched pairs would be noted on the datasheet. This ensures the antibodies are detecting ...

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Thank you for your inquiry. Unfortunately, the isoelectric points of these antibodies have not been determined. According to my research, the pI of most monoclonal antibodies is around 6.1. The isoelectric point for horseradish peroxidase isozyme...

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That is correct: ab10184 for capture and ab24466 for detection. These are two different clones.  The detection antibody, ab24466, is conjugated to HRP. Some of the catalogue numbers of our other PSA antibodies are very similar so I will not even mentio...

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Thank you for your enquiry. Anti-PSA MAbs do not react with linear peptides, they react only with conformational peptides. Epitope groups are determined in "ISOBM TD-3 International Workshop on Monoclonal Antibodies against Prostate-Specific Antigen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"