Prostate Specific Antigen antibody [A67-B/E3] (ab2218)

Product Name 

Prostate Specific Antigen antibody [A67-B/E3]

Product type 

Primary antibodies

Description 

Mouse monoclonal [A67-B/E3] to Prostate Specific Antigen

Immunogen 

Tissue / cell preparation (Human). 30 kDa protein fraction prepared from human seminal plasma.

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific for PSA.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependant dilution.
IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Secreted

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Adhesion Proteins >> Proteases

Relevance 

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

A67-B/E3

Isotype 

IgG1

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites using precipitation methods.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Prostate Specific Antigen antibody [A67-B/E3] (ab2218)

  This product has been used in:

Rye P.D.et al. (eds.) Summary Report on the ISOBM TD-3 Workshop. Tum. Biol. 20:1-94 (1999). If you publish research using ab2218 please let us know so that we can cite the reference on this datasheet.

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