Recombinant Anti-Prostate Specific Antigen antibody [EP1588Y] - Low endotoxin, Azide free (ab215983)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen - Low endotoxin, Azide free
- Suitable for: mIHC, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Prostate Specific Antigen antibody [EP1588Y] - Low endotoxin, Azide free
See all Prostate Specific Antigen primary antibodies -
Description
Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen - Low endotoxin, Azide free -
Host species
Rabbit -
Specificity
The antibody provided good results in western blot when tested in human prostate lysate. Unfortunately, when 22RV1 cell lysate was used no band was detected and inconsistent results were obtained with LNCaP cell lysate (where different lots of cell lysate were used for the test). Please contact our Scientific Support if you have any feedback and/or questions.
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Tested applications
Suitable for: mIHC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human prostate carcinoma tissue lysate. IHC-P: Human prostate cancer tissue.
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General notes
ab215983 is the carrier-free version of ab76113.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 5.04 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1588Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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mIHC |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
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Notes |
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mIHC
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
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Alternative names
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
Images
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This data was developed using ab76113, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate cancer tissue sections labeling Prostate Specific Antigen with purified ab76113 at 1/1000 dilution (0.51 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76113).
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (5)
ab215983 has been referenced in 5 publications.
- Volpetti F et al. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS One 10:e0117744 (2015). PubMed: 25680117
- Barber AG et al. Characterization of desmoglein expression in the normal prostatic gland. Desmoglein 2 is an independent prognostic factor for aggressive prostate cancer. PLoS One 9:e98786 (2014). Human . PubMed: 24896103
- Shiota M et al. Inhibition of protein kinase C/Twist1 signaling augments anticancer effects of androgen deprivation and enzalutamide in prostate cancer. Clin Cancer Res 20:951-61 (2014). PubMed: 24352647
- Srivastava SK et al. Myb overexpression overrides androgen depletion-induced cell cycle arrest and apoptosis in prostate cancer cells, and confers aggressive malignant traits: potential role in castration resistance. Carcinogenesis 33:1149-57 (2012). PubMed: 22431717
- Morrissey C et al. Effect of artemisinin derivatives on apoptosis and cell cycle in prostate cancer cells. Anticancer Drugs 21:423-32 (2010). PubMed: 20130467