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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prostate Specific Antigen antibody [PSA1] (ab403)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prostate Specific Antigen antibody [PSA1] (ab403)](/ps/datasheet/images/0/ab403/Prostate-Specific-Antigen-Primary-antibodies-ab403-5.JPG)
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Product Name
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Prostate Specific Antigen antibody [PSA1]
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See all Prostate Specific Antigen antibodies (32)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [PSA1] to Prostate Specific Antigen
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Immunogen
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native protein (purified), corresponding to amino acids 158-163 of Human Prostate Specific Antigen
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Reacts with
(species key)
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Hu
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Specificity
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Human prostate-specific antigen - 100%
PSA/alpha-1-ACT-complex - 59%
Kallikrein - <0.04%
PAP - <0.04%
Cross-reactivity values have been calculated on weight/weight basis using 125-I-labelled human human prostate-specific antigen in assay. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays.
Affinity constant: 1x1011 l/mol human prostate-specific antigen.
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Tested applications
(see key)
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ELISA, IHC-P, RIA
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependant concentration.
IHC-P: Use at an assay dependent dilution.
RIA: Use at an assay dependant concentration.
Nanowires array: 1/50 - 1/500.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Secreted
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Research areas
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Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Adhesion Proteins >> Proteases
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Relevance
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Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.
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Prostate Specific Antigen antibody [PSA1] (ab403) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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PSA1
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Sodium Chloride, Citrate / phosphate. pH 6.0 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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5.300 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Prostate Specific Antigen antibody [PSA1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Prostate Specific Antigen antibody [PSA1] images, references, products related to ab403 and other tools.
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Prostate Specific Antigen antibody [PSA1] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prostate Specific Antigen antibody [PSA1] (ab403)
Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for Prostate Specific Antigen antibody [PSA1] (ab403)
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This product has been used in: (two most recent references)
Thaxton CS et al. Nanoparticle-based bio-barcode assay redefines "undetectable" PSA and biochemical recurrence after radical prostatectomy. Proc Natl Acad Sci U S A 106:18437-42 (2009). Human. PubMed: 19841273
Zheng G et al. Multiplexed electrical detection of cancer markers with nanowire sensor arrays. Nat Biotechnol 23:1294-301 (2005). PubMed: 16170313
If you publish research using ab403 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Prostate Specific Antigen
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Prostate Specific Antigen antibody [PSA1] - more information
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Customer reviews (feedback) regarding Prostate Specific Antigen antibody [PSA1] |
Customer FAQs regarding Prostate Specific Antigen antibody [PSA1] |
Protocols for Prostate Specific Antigen antibody [PSA1] |
Price and availability of products related to Prostate Specific Antigen antibody [PSA1] |
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Prostate Specific Antigen antibody [PSA1] - related products:
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Prostate Specific Antigen antibody [PSA1] - other tools:
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Contact Abcam with a Technical Enquiry about Prostate Specific Antigen antibody [PSA1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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