![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prostate Specific Antigen antibody [PSA1] (ab403) image](#Prostate-Specific-Antigen-Primary-antibodies-ab403-5.JPG)
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Products:Neuroscience >> Cell Adhesion Proteins >> Proteases
Overview
Product name
Anti-Prostate Specific Antigen antibody [PSA1]
See all Prostate Specific Antigen products (32) ...
Description
Mouse monoclonal [PSA1] to Prostate Specific Antigen
Specificity
Human prostate-specific antigen - 100% PSA/alpha-1-ACT-complex - 59% Kallikrein - <0.04% PAP - <0.04% Cross-reactivity values have been calculated on weight/weight basis using 125-I-labelled human human prostate-specific antigen in assay. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays. Affinity constant: 1x1011 l/mol human prostate-specific antigen.
Tested applications
ELISA, RIA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
native protein (purified), corresponding to amino acids 158-163 of Human Prostate Specific Antigen
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.095% Sodium Azide
Constituents: 0.9% Sodium chloride, 37mM Sodium citrate, 125mM PBS, pH 6.0
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
PSA1
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Adhesion Proteins >> Proteases
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prostate Specific Antigen antibody [PSA1] (ab403)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prostate Specific Antigen antibody [PSA1] (ab403) image](/ps/datasheet/images/0/ab403/Prostate-Specific-Antigen-Primary-antibodies-ab403-5.JPG)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
RIA
Application notes
ELISA: Use at an assay dependant concentration.
IHC-P: Use at an assay dependent dilution.
RIA: Use at an assay dependant concentration.
Nanowires array: 1/50 - 1/500.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionP07288
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APS antibody
- Gamma seminoprotein antibody
- Gamma-seminoprotein antibody
see all
Database links
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
Anti-Prostate Specific Antigen antibody [PSA1] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prostate Specific Antigen antibody [PSA1] (ab403)
Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Prostate Specific Antigen antibody [PSA1] (ab403)
This product has been referenced in:
- Shu SKet al. Phosphorylation and activation of androgen receptor by Aurora-A. J Biol Chem 285:33045-53 (2010). WB; Human.Read more (PubMed: 20713353) »
- Thaxton CSet al. Nanoparticle-based bio-barcode assay redefines "undetectable" PSA and biochemical recurrence after radical prostatectomy. Proc Natl Acad Sci U S A 106:18437-42 (2009). Human.Read more (PubMed: 19841273) »
See all 3 publications for this product
Publishing research using ab403? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"