Anti-Prostate Specific Antigen antibody (ab9537)

Overview

  • Product nameAnti-Prostate Specific Antigen antibody
    See all Prostate Specific Antigen primary antibodies
  • Description
    Rabbit polyclonal to Prostate Specific Antigen
  • SpecificityThis antibody reacts with PSA. Staining was observed in the prostatic ductal epithelium of normal and neoplastic prostate. No staining with any other cell type has been observed.
  • Tested applicationsIHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Prostate specific antigen isolated from human seminal plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab9537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/150.
IHC-Fr Use at an assay dependent dilution.
ICC/IF 1/1000.

Target

  • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • KLK3 antibody
    • KLK3_HUMAN antibody
    • P 30 antigen antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate Specific Antigen antibody
    • Prostate Specific Antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

Anti-Prostate Specific Antigen antibody images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue, staining Prostate Specific Antigen with ab9537. Staining was detected using DAB.

References for Anti-Prostate Specific Antigen antibody (ab9537)

This product has been referenced in:
  • Sanz G  et al. Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation. PLoS One 9:e85110 (2014). IHC (PFA fixed) ; Mouse . Read more (PubMed: 24416348) »
  • Berry SM  et al. Streamlining immunoassays with immiscible filtrations assisted by surface tension. Anal Chem 84:5518-23 (2012). Read more (PubMed: 22632629) »

See all 4 Publications for this product

Product Wall

Thank you for contacting Abcam regarding ab9537.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the...

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Thank you for contacting Abcam regarding ab9537.


This antibody has been validated for IF, however currently we do not have any references available in which a customer has published this data.


I have forwarded your quote req...

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Thank you for your enquiry and interest.

Could you please clarify and confirm in what type of assay/application your customer is looking for a primary antibody and antigen? This would be important for me to know to be able to suggest the best...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (LNCaP Cells)
Specification LNCaP Cells
Fixative Methanol
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Apr 16 2007

Thank you for your email. I'm replying on behalf of Charlotte who is currently away from the office. To clarify the concentration of this product, the concentration of the anti-PSA IgG is 100 ug/ml. This antibody has not been affinity purified (the ...

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Thank you for your enquiry. Following correspondence with the lab I have determined that the total protein conc. of this antibody is 1.5 mg/ml but the IgG conc. is 100 ug/ml. Please can you confirm that you mean 100 ng/ml and not 100 ug/ml.

Thank you for your email. I can't tell you the name of the originator of ab9537 as that information is proprietary. I double-checked ab403 and ab2218, and both of these do not contain BSA or other proteins in the storage buffer. Ab403 consists of p...

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Thank you for your email. I checked with the antibody's originator again and the antibody is suspended in PBS which contains BSA. I apologize as this information was not mentioned before. I have now added it to the online datasheet. Please contact u...

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Thank you for your email. The antibody storage buffer consists of PBS with 0.05% Sodium Azide. Please contact us again if you have any additional questions.

Thank you for your enquiry. The antibody is suspended in PBS and stains ductal epithelium. We do not know if glandular epithelium is also stained. Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"