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Proteasome 19S S4 protein (Tagged-His Tag) (ab91949)

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Overview

  • Product nameProteasome 19S S4 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 225-440 (216 amino acids) of Human Proteasome 19S S4 with N terminal His tag; Predicted MWt 25 kDa (UniProt P62191).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 77 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGP KLVRELFRV AEEHAPSIVFIDEIDAIGTKRYDSNSGGE REIQRTMLELLNQLDGFDSR GDVKVIMATNRIETLDPA LIRPGRIDRKIEFPLPDEKTKKRIFQIHTSR MTLADDV TLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNED FK KSKENVLYKKQEGTPEGLYL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91949 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        26S protease regulatory subunit 426S proteasome AAA-ATPase subunit RPT2MGC24583
        MGC8541P26S4p56Proteasome (prosome macropain) 26S subunit ATPase 1Proteasome 26S ATPase subunit 1Proteasome 26S subunit ATPase 1PRS4_HUMANPSMC 1PSMC1S4
      see all
  • FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
  • Sequence similaritiesBelongs to the AAA ATPase family.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession P62191 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Proteasome 19S S4 protein (Tagged-His Tag) images

    • SDS-PAGE - Proteasome 19S S4 protein (Tagged-His Tag) (ab91949)
      SDS-PAGE - Proteasome 19S S4 protein (Tagged-His Tag) (ab91949)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Proteasome 19S S4 protein (Tagged-His Tag) (ab91949)

    ab91949 has not yet been referenced specifically in any publications.

    Publishing research using ab91949 ? Please let us know so that we can cite the reference in this datasheet

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