Anti-MSS1 antibody (ab233127)
Key features and details
- Rabbit polyclonal to MSS1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MSS1 antibody
See all MSS1 primary antibodies -
Description
Rabbit polyclonal to MSS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Orangutan -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human MSS1 aa 1 to the C-terminus. Two N-terminal tags. Expressed in E.coli.
Database link: P35998 -
Positive control
- WB: Recombinant human MSS1 protein; Mouse spleen and liver lysates; U-2 OS cell lysate. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 49 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 49 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation. -
Sequence similarities
Belongs to the AAA ATPase family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5701 Human
- Entrez Gene: 19181 Mouse
- Entrez Gene: 25581 Rat
- Entrez Gene: 399327 Xenopus laevis
- Omim: 154365 Human
- SwissProt: Q5E9F9 Cow
- SwissProt: P35998 Human
- SwissProt: P46471 Mouse
see all -
Alternative names
- 26S protease complex antibody
- 26S protease regulatory subunit 7 antibody
- 26S proteasome AAA-ATPase subunit RPT1 antibody
see all
Images
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Anti-MSS1 antibody (ab233127) at 2 µg/ml + Recombinant human Proteasome 19S S7 protein.
Predicted band size: 49 kDa -
Anti-MSS1 antibody (ab233127) at 2 µg/ml + Mouse spleen tissue lysate
Predicted band size: 49 kDa -
Anti-MSS1 antibody (ab233127) at 2 µg/ml + U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate
Predicted band size: 49 kDa -
Anti-MSS1 antibody (ab233127) at 2 µg/ml + Mouse liver tissue lysate.
Predicted band size: 49 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for MSS1 using ab233127 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab233127 has been referenced in 1 publication.
- Zhou B et al. Silencing Proteasome 26S Subunit ATPase 2 (PSMC2) Protects the Osteogenic Differentiation In Vitro and Osteogenesis In Vivo. Calcif Tissue Int 109:44-54 (2021). PubMed: 33625534