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All lanes : Anti-Proteasome 19S S7 antibody (ab49058) at 1.25 µg/ml
Lane 1 : MARKER
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 49 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
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