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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
Overview
Product name
Anti-Proteasome 19S S7 antibody
See all Proteasome 19S S7 products (5) ...
Description
Rabbit polyclonal to Proteasome 19S S7
Specificity
This antibody is specific for Proteasome 19S S7
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Zebrafish
Immunogen
A region within synthetic peptide: MPDYLGADQR KTKEDEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED, corresponding to N terminal amino acids 1-50 of Human Proteasome 19S S7
MPDYLGADQR KTKEDEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED
Positive control
Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
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Western blot - Proteasome 19S S7 antibody (ab49058)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).
Target
Function
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation.
Sequence similarities
Belongs to the AAA ATPase family.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP35998
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 26S protease complex antibody
- 26S protease regulatory subunit 7 antibody
- 26S proteasome AAA-ATPase subunit RPT1 antibody
see all
Database links
- Entrez Gene: 5701 Human
- Entrez Gene: 19181 Mouse
- Entrez Gene: 25581 Rat
- Omim: 154365 Human
- SwissProt: P35998 Human
- SwissProt: P46471 Mouse
- SwissProt: Q63347 Rat
- Unigene: 437366 Human
see all
Anti-Proteasome 19S S7 antibody images:
Western blot - Proteasome 19S S7 antibody (ab49058)
All lanes : Anti-Proteasome 19S S7 antibody (ab49058) at 1.25 µg/ml
Lane 1 : MARKER
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 49 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
References for Anti-Proteasome 19S S7 antibody (ab49058)
ab49058 has not yet been referenced specifically in any publications.
Publishing research using ab49058? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"