Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab189855)
Key features and details
- Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 20S alpha 5/PSMA5 antibody
See all Proteasome 20S alpha 5/PSMA5 primary antibodies -
Description
Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant full length protein corresponding to Human Proteasome 20S alpha 5/PSMA5 aa 1 to the C-terminus.
Database link: P28066 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 26 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 26 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
Tissue specificity
Expressed in fetal brain (at protein level). -
Sequence similarities
Belongs to the peptidase T1A family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 510155 Cow
- Entrez Gene: 5686 Human
- Entrez Gene: 26442 Mouse
- Omim: 176844 Human
- SwissProt: Q5E987 Cow
- SwissProt: P28066 Human
- SwissProt: Q9Z2U1 Mouse
- Unigene: 485246 Human
see all -
Alternative names
- Macropain zeta chain antibody
- Multicatalytic endopeptidase complex zeta chain antibody
- Proteasome (prosome macropain) subunit alpha type 5 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab189855. Blue DAPI for nuclear staining.
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All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab189855) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : 293T cell extract
Lane 3 : HepG2 cell extract
Lane 4 : U-251MG cell extract
Lane 5 : BxPC3 cell extract
Lane 6 : Mouse liver extract
Lane 7 : Mouse thymus extract
Lane 8 : Mouse heart extract
Predicted band size: 26 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab189855 has been referenced in 2 publications.
- Morrow CS et al. Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit. Cell Stem Cell 26:558-568.e9 (2020). PubMed: 32109376
- Schmidt H et al. IL-13 Impairs Tight Junctions in Airway Epithelia. Int J Mol Sci 20:N/A (2019). PubMed: 31262043