Anti-Proteasome 20S beta 6 antibody (ab3331)
Key features and details
- Rabbit polyclonal to Proteasome 20S beta 6
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Proteasome 20S beta 6 antibody
See all Proteasome 20S beta 6 primary antibodies -
Description
Rabbit polyclonal to Proteasome 20S beta 6 -
Host species
Rabbit -
Specificity
Detects proteasome 20S beta 6 from purified bovine and human 26S proteasome samples. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Predicted to work with: Xenopus laevis, Zebrafish -
Immunogen
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Positive control
- WB: HeLa, BAEC whole cell lysates, HeLa, 3T3-L1, PC-3, MCF7, A549, PANC-1, Mouse Kidney, Mouse Liver and Rat Liver nuclear enriched cell lysate. ICC/IF: A431, HeLa, NIH 3T3, BAEC cells.
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General notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/10 - 1/100.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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Notes |
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ICC/IF
1/10 - 1/100. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens. -
Sequence similarities
Belongs to the peptidase T1B family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5694 Human
- Entrez Gene: 19175 Mouse
- Entrez Gene: 100360846 Rat
- Entrez Gene: 100364558 Rat
- Entrez Gene: 29666 Rat
- Entrez Gene: 30388 Zebrafish
- Omim: 600307 Human
- SwissProt: P28072 Human
see all -
Alternative names
- DELTA antibody
- LMPY antibody
- Macropain delta chain antibody
see all
Images
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All lanes : Anti-Proteasome 20S beta 6 antibody (ab3331) at 1 µg/ml
Lane 1 : Nuclear enriched extracts from untransfected PC-3 cells
Lane 2 : Nuclear enriched extracts from non-targeting scrambled siRNA transfected PC-3 cells
Lane 3 : Nuclear enriched extracts from PSMB6 knockdown PC-3 cells
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/6000 dilution
Predicted band size: 25 kDa -
All lanes : Anti-Proteasome 20S beta 6 antibody (ab3331) at 1 µg/ml
Lane 1 : HeLa nuclear enriched extract lysate
Lane 2 : 3T3-L1 nuclear enriched extract lysate
Lane 3 : PC-3 nuclear enriched extract lysate
Lane 4 : MCF7 nuclear enriched extract lysate
Lane 5 : A549 nuclear enriched extract lysate
Lane 6 : PANC-1 nuclear enriched extract lysate
Lane 7 : Mouse Kidney nuclear enriched extract lysate
Lane 8 : Mouse Liver nuclear enriched extract lysate
Lane 9 : Rat Liver nuclear enriched extract lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/6000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?Samples were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel and the resolved proteins were then transferred onto a Nitrocellulose membrane by iBlot® 2 Dry Blotting System. The blot was probed with the primary antibody (ab3331) and detected by chemiluminescence with secondary antibody using the iBright FL 1000. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit. -
All lanes : Anti-Proteasome 20S beta 6 antibody (ab3331) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 2 : BAEC (Bovine aortic endothelial cell line) whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Predicted band size: 25 kDa -
Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of A431 (Human epidermoid carcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunofluorescent analysis of Proteasome 20S Y (green) in NIH/3T3 (Mouse embryo fibroblast cell line) cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes and blocked with 3% Blocker BSA in PBS for 30 minutes at room temperature. Cells were stained with or without Proteasome 20S Y rabbit polyclonal antibody, at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at 1/1000 dilution for 1 hour at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
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Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of BAEC (Bovine aortic endothelial cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of HeLa (Human epithelial adenocarcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab3331 has been referenced in 1 publication.
- de Verteuil D et al. Deletion of immunoproteasome subunits imprints on the transcriptome and has a broad impact on peptides presented by major histocompatibility complex I molecules. Mol Cell Proteomics 9:2034-47 (2010). WB ; Mouse . PubMed: 20484733