Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Proteasome 20S beta 7 antibody
See all Proteasome 20S beta 7 products (4) ...
Description
Chicken polyclonal to Proteasome 20S beta 7
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to C terminal amino acids 151-277 of Human Proteasome 20S beta2.
Positive control
Proteasome 20S beta2 fusion protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
Signal Transduction >> Metabolism >> Amino Acids
-
Western blot - Proteasome 20S beta2 antibody (ab37616)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/2000. Predicted molecular weight: 30 kDa.
We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
Tissue specificity
Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues.
Sequence similarities
Belongs to the peptidase T1B family.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ99436
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Macropain chain Z antibody
- Multicatalytic endopeptidase complex chain Z antibody
- Proteasome (prosome macropain) subunit beta type 7 antibody
see all
Database links
- Entrez Gene: 5695 Human
- Entrez Gene: 19177 Mouse
- Entrez Gene: 85492 Rat
- Omim: 604030 Human
- SwissProt: Q99436 Human
- SwissProt: P70195 Mouse
- SwissProt: Q9JHW0 Rat
- Unigene: 213470 Human
see all
Anti-Proteasome 20S beta 7 antibody images:
Western blot - Proteasome 20S beta2 antibody (ab37616)
Anti-Proteasome 20S beta 7 antibody (ab37616) at 1/2000 dilution + E coli derived fusion protein
Secondary
Goat anti IgY HRP at 1/1000 dilution
developed using the ECL technique
Predicted band size : 30 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the Proteasome 20S beta2 fusion protein.
Predicted and actual molecular weights differ due to the use of the fusion protein.
References for Anti-Proteasome 20S beta 7 antibody (ab37616)
ab37616 has not yet been referenced specifically in any publications.
Publishing research using ab37616? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"