Anti-Proteasome 26S S3/PSMD3 antibody (ab154939)
Key features and details
- Rabbit polyclonal to Proteasome 26S S3/PSMD3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 26S S3/PSMD3 antibody
See all Proteasome 26S S3/PSMD3 primary antibodies -
Description
Rabbit polyclonal to Proteasome 26S S3/PSMD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish -
Immunogen
Recombinant fragment corresponding to Human Proteasome 26S S3/PSMD3 aa 305-534.
Database link: O43242 -
Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji whole cell lysates; A431 cells.
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General notes
Keep as concentrated solution.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
Sequence similarities
Belongs to the proteasome subunit S3 family.
Contains 1 PCI domain. - Information by UniProt
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Database links
- Entrez Gene: 426133 Chicken
- Entrez Gene: 504937 Cow
- Entrez Gene: 5709 Human
- Entrez Gene: 22123 Mouse
- Entrez Gene: 287670 Rat
- Entrez Gene: 444384 Xenopus laevis
- Entrez Gene: 393544 Zebrafish
- SwissProt: Q2KJ46 Cow
see all -
Alternative names
- 26S proteasome non ATPase regulatory subunit 3 antibody
- 26S proteasome non-ATPase regulatory subunit 3 antibody
- 26S proteasome regulatory subunit RPN3 antibody
see all
Images
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All lanes : Anti-Proteasome 26S S3/PSMD3 antibody (ab154939) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Hep G2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed A431 cells labeling Proteasome 26S S3/PSMD3 with ab154939 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154939 has not yet been referenced specifically in any publications.