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Proteasome subunit beta type 2 protein (Tagged-His Tag) (ab91716)

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Overview

  • Product nameProteasome subunit beta type 2 protein (Tagged-His Tag)See all Proteasome subunit beta type 2 proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-201 of Human Proteasome subunit beta type 2 with N terminal His tag, 21kDa. UniProt ID = P49721.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 115 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesMEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSE KILLLCVGEAGD TVQFAEYIQKNVQLYKMRNGYELSPT AAANFTRRNLADCLRSRTPYHVNLLL AGYDEHEGPALY YMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERA VELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNI SFPKQGS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91716 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        HC7 IMacropain subunit C7 IMacropain subunit C7-I
        Multicatalytic endopeptidase complex subunit C7 1Multicatalytic endopeptidase complex subunit C7 IMulticatalytic endopeptidase complex subunit C7-IProteasome (prosome, macropain) subunit beta type 2Proteasome beta 2 subunitProteasome component C7 IProteasome component C7-IProteasome subunit beta type-2PSB2_HUMANPSMB2
      see all
  • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
  • Sequence similaritiesBelongs to the peptidase T1B family.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession P49721 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Proteasome subunit beta type 2 protein (Tagged-His Tag) images

    • SDS-PAGE - Proteasome subunit beta type 2 protein (Tagged-His Tag) (ab91716)
      SDS-PAGE - Proteasome subunit beta type 2 protein (Tagged-His Tag) (ab91716)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Proteasome subunit beta type 2 protein (Tagged-His Tag) (ab91716)

    ab91716 has not yet been referenced specifically in any publications.

    Publishing research using ab91716 ? Please let us know so that we can cite the reference in this datasheet

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