Overview
- Product nameAnti-Protein A antibody (Biotin)See all Protein A primary antibodies ...
- DescriptionGoat polyclonal to Protein A (Biotin)
- ConjugationBiotin
- Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
- SpecificityNo reaction is observed against Protein G.
- Tested applicationsDot Blot, ELISA, IHC-Fr, Immunomicroscopy, WB more details
- Immunogen
Protein A [Staphylococcus aureus]
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
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Concentration information loading... - PurityIgG fraction
- Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab7246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Dot Blot | |
| ELISA | |
| IHC-Fr | |
| Immunomicroscopy | |
| WB |
Target
- RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
- Cellular localizationSecreted; cell wall.
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Alternative names
- gG-binding protein A antibodyImmunoglobulin G binding protein A antibodySPA antibodyStaphylococcal protein A antibody
References for Anti-Protein A antibody (Biotin) (ab7246)
ab7246 has not yet been referenced specifically in any publications.
