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Anti-Protein A antibody (HRP) (ab18596)

Submit an Abreview Q&A (11)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES. pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgY
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab18596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
ELISA ELISA: 1/5000 - 1/20000.

Target

  • RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationSecreted; cell wall.
  • Alternative names
      gG-binding protein A antibodyImmunoglobulin G binding protein A antibodySPA antibodyStaphylococcal protein A antibody

References for Anti-Protein A antibody (HRP) (ab18596)

ab18596 has not yet been referenced specifically in any publications.

Publishing research using ab18596 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab18596 Submit a Question for ab18596

Displaying 1 - 10 of 11 results for Abreviews and Q&A

10
Votes: Highest First

Could you please send me expiry dating and data on the following products/lots? Chicken polyclonal antibody to Protein A (1 mg/mL from Abcam Cat. # ab19483) Lot: GR58389-4 Chicken polyclonal antibody to Protein A (HRP) (1 mg/mL from Abcam Cat. # ab18596...

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We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (http://www.abcam.com/index.html?pagecon...

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Dear Madam or Sir, in a previous request I asked for the availability of an antibody and got the answer below. "For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vials of different stocks of this antibody in the UK....

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Thank you for contacting us.

Worldwide, we have 16 vials of this antibody in stock. We do not have more as it is not a bestseller, but sells quite solid. This product is not a niche product either, therefore, we constantly have have a certain thr...

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Dear Madam or Sir, we would be interested in knowing how many vials of the product no 19483, lots GR58389 - X and prod no 18596 lots GR74278 - X are still available. Is it correct that the last number of the lot (indicated here as X) indicates the filling...

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Thank you for contacting us.

Yes, you are right. The number after the dash signifies when the lot was aliquoted. If the number directly after the "GR" is the same then it is the same lot.

For ab19483, chicken polyclonal to P...

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Inquiry: Dear Madam or Sir, we would be interested in knowing how many vials of the product no 19483, lots GR58389 - X and prod no 18596 lots GR74278 - X are still available. Is it correct that the last number of the lot (indicated here as X) indicates th...

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Thank you for contacting us.

We currently have x vials of ab19483, lot GR58389 worldwide (x in UK office, x in US office, x in Japan office.)

For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vials o...

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Inquiry: I want to know if protA EIAs from a specific lot number are still available, Chk pAb anti-Protein A, ab19483, GR58389-2 AND Chk pAb anti-Protein A - HRP, ab18596, GR74278-2.

Thank you for your enquiry and your interest in our products.

I have checked the stock viability and can confirm the followings:

- ab19483 - several vials from the same bleed and purification;

- ab18596 - only one vial left f...

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Currently we are working with two of your antibodies: Chicken polyclonal to Protein A (ab19483) and Anti-Protein A antibody-HRP (ab18596). On your product sheet I found that the Chicken polyclonal to Protein A is IgY an the Anti-Protein A antibody-HRP is ...

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Thank you for contacting us. I apologize for being late in getting back to you.

I can confirm that both antibodies ab18596 and ab19483 have similar IgY isotype.

I hope this information is helpful to you. Please do not hesitate to contact...

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I confirmed that order number is ###### of ab18596(lot.GR27429-2) and he purchased 33 vials of this antibody so far.
He understood about guarantee duration but I think he knows well this antibody and if there's some problem, he needs to get compensat...

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Thank you for confirming this information and for your help and cooperation with this case.

As a special exception as the customer has used so many vials succesfully, we can extend the guarantee period in this case and Iasked our accounting dep...

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Our customer obtained different result from ab18596 despite in the same conditions so,

Please arrange a credit note.

And he would like to receive CoA of two vials


Ab18596-1/ lot# 793467

Ab18596/lot# GR2742...

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Thank you for your reply.

I appreciate your cooperation and understand your concerns. It is regrettable the results from lot 793467have not been assatisfactory for the customer. I can confirm that ab18596 and ab18596-1 are the same antibody. Th...

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Dear technical team,


Our customer purchased ab18596-1(lot.793467) and ab18596(lot.GR27429-2).

He would like to know if they have differences (I have already explained about lot# difference) because when he tested lot.793467, de...

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Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from this antibody.

As this is a polyclonal antibody, some batch variability could be expected. It is possible there could also be vari...

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I just purchased 3 of your chicken anti-Protein A antibodies, ab18596 (HRP-labeled), ab18598 (Biotin-labeled), and ab19483 (unconjugated). We were wondering, are these all the same antibody, or are the labeled antibodies different that the unconjugated on...

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Thank you for contacting us.

The conjugated antibodies ab18596 and ab18598 are prepared from the unconjugated ab19483. The formulations differ (for instance, there is no azide in the HRP conjugate buffer) but the antibody is the same for all th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"