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Overview

  • Product nameProtein Block
  • Tested applicationsIHC-P, ELISA, WB more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.5% BSA, 0.5% Casein, PBS
  • Concentration information loading...
  • Reagent notes ab64226 reduces the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. It has been shown to be effective for immunohistochemistry, ELISA, and blot and requires no mixing or diluting.
  • Research Areas
  • RelevanceProtein Block is used with immunolabeling techniques for the reduction of non-specific background staining.

    • Applications

    Our Abpromise guarantee covers the use of ab64226 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution.
    ELISA ELISA: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.

    References for Protein Block (ab64226)

    This product has been referenced in:
    • Takeda K  et al. Expression of LGR5, an intestinal stem cell marker, during each stage of colorectal tumorigenesis. Anticancer Res 31:263-70 (2011). Read more (PubMed: 21273608) »

    See 1 Publication for this product

    Publishing research using ab64226 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab64226 Submit a Question for ab64226

    Displaying 1 - 3 of 3 results for Abreviews and Q&A

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    ab128988:
    What is the compositions of the protein block? Does it contain BSA?
    Can the block be used in ICC?

    Thank you for contacting us.
    The lab sent me the following information:
    1. What is the compositions of the protein block? Does it contain BSA?
    ANSWER: PBS solution, pH 7.6, with 0.5% BSA, 0.5% casein and less than 0.1% sodium azide
    2. ...

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    I need to develop double IHC analysis on formalin-fixed paraffin-embedded (FFPE) tissue sections of human tumors specimens, and after we have to do laser capture microdissection of positive cells.   I already have two primary antibodies directed against...

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    Thank you for contacting us.

    We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT is ...

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    Last week I asked Janice White, who is responsible for the purchasing, to order an Abcam antiboty (CD31, ab28364) and protein block (ab64226). But the products were delivered last Friday when she was not in her office  and she gave me an email on the arri...

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    Most of our antibodies will remain stable for 7 days at room temperature. For ab28364, anti-CD31, it should still be viable and work as expected. As for the protein block, we have no information on how long it will remain viable at room temperature, but I...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"