Overview

  • Product nameProtein Block
  • Tested applicationsIHC-P, ELISA, WB more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.5% BSA, 0.5% Casein, PBS
  • Concentration information loading...
  • Reagent notes ab64226 reduces the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. It has been shown to be effective for immunohistochemistry, ELISA, and blot and requires no mixing or diluting.
  • Research Areas
  • RelevanceProtein Block is used with immunolabeling techniques for the reduction of non-specific background staining.

Applications

Our Abpromise guarantee covers the use of ab64226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

References for Protein Block (ab64226)

This product has been referenced in:
  • Takeda K  et al. Expression of LGR5, an intestinal stem cell marker, during each stage of colorectal tumorigenesis. Anticancer Res 31:263-70 (2011). Read more (PubMed: 21273608) »

See 1 Publication for this product

Product Wall

ab64226 is the protein block used in ab80437. A link to ab64226 is below:

Thank you for contacting us.
The lab sent me the following information:
1. What is the compositions of the protein block? Does it contain BSA?
ANSWER: PBS solution, pH 7.6, with 0.5% BSA, 0.5% casein and less than 0.1% sodium azide
...

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Thank you for contacting us.

We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT ...

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Most of our antibodies will remain stable for 7 days at room temperature. For ab28364, anti-CD31, it should still be viable and work as expected. As for the protein block, we have no information on how long it will remain viable at room temperature, bu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"