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Predicted band size : 52 kDa
APC and PNGase F-treated APC (PNG-APC, 1 μg) were characterized by 4–15% SDS-PAGE analysis and Western blotting, using ab28663, that detects the protein C/APC heavy chain (HC, shown). For wild type APC, a doublet of ∼35–40 kDa, corresponding to the heavy chains of the two major APC glycoforms, α and β, was identified. PNGase F treatment of APC reduced the molecular mass of the APC doublet ∼10 kDa, corresponding to the disappearance of the fully glycosylated APC heavy chain and the formation of lower molecular mass APC glycoforms upon N-linked glycan proteolysis
Image from Ní Ainle F et al, J Biol Chem. 2011 Jan 14;286(2):1323-30. Epub 2010 Nov 2, Fig 1. DOI 10.1074/jbc.M110.159475
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