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Products:Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Overview
Product name
Protein C protein
See all Protein C products (7) ...
Protein description
Purified full length native human Protein C protein (MW 62kDa) prepared from fresh frozen citrated plasma. Post-translational modifications: nine gla residues (human), one beta hydroxyaspartate
Properties
Purity
Immunogen affinity purified
Purification notes
ab62448 is purified by a combination of immunoaffinity chromatography, and conventional techniques. Purity is determined by SDS-PAGE analysis.
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Additional notes
Concentration varies from lot to lot but is between 5-10mg/ml
Research areas
Cardiovascular >> Blood >> Coagulation >> Regulatory
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
FuncS: Use at an assay dependent dilution. (Use at an assay dependent dilution. Note: activity is measured using a chromogenic substrate based assay.)
Protein info
Function
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids.
Tissue specificity
Plasma; synthesized in the liver.
Involvement in disease
Defects in PROC are the cause of protein C deficiency autosomal dominant (ADPROCD) [MIM:176860]. ADPROCD is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Individuals with decreased amounts of protein C are classically referred to as having type I protein C deficiency and those with normal amounts of a functionally defective protein as having type II deficiency.
Defects in PROC are the cause of protein C deficiency autosomal recessive (ARPROCD) [MIM:612304]. ARPROCD results in a thrombotic condition that can manifest as a severe neonatal disorder or as a milder disorder with late-onset thrombophilia. The severe form leads to neonatal death through massive neonatal venous thrombosis. Often associated with ecchymotic skin lesions which can turn necrotic called purpura fulminans, this disorder is very rare.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
Post-translational
modifications
The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not glycosylated at Asn-371, is beta.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Target information above from: UniProt accessionP04070
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Protein C protein (ab62448)
ab62448 has not yet been referenced specifically in any publications.
Publishing research using ab62448? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"