Anti-Protein G antibody (ab181700)
Key features and details
- Rabbit polyclonal to Protein G
- Suitable for: IHC-P, WB
- Reacts with: Streptococcus sp. group G
- Isotype: IgG
Overview
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Product name
Anti-Protein G antibody
See all Protein G primary antibodies -
Description
Rabbit polyclonal to Protein G -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Streptococcus sp. group G -
Immunogen
Full length native protein (purified) corresponding to Protein G aa 34-562. Streptococcus species.
Sequence:VDSPIEDTPIIRNGGELTNLLGNSETTLALRNEESATADLTAAAVADTVA AAAAENAGAAAWEAAAAADALAKAKADALKEFNKYGVSDYYKNLINNAKT VEGVKDLQAQVVESAKKARISEATDGLSDFLKSQTPAEDTVKSIELAEAK VLANRELDKYGVSDYHKNLINNAKTVEGVKDLQAQVVESAKKARISEATD GLSDFLKSQTPAEDTVKSIELAEAKVLANRELDKYGVSDYYKNLINNAKT VEGVKALIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQ YANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTL KGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVID ASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGV WTYDDATKTFTVTEMVTEVPGDAPTEPEKPEASIPLVPLTPATPIAKDDA KKDDTKKEDAKKPEAKKEDAKKAETLPTT
Database link: P19909 -
Positive control
- Protein G
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.27% Tripotassium orthophosphate -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab181700 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer it is provided in. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 63 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 63 kDa. |
Target
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Relevance
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Cell Wall and Secreted -
Alternative names
- IgG binding protein G antibody
- IgG-binding protein G antibody
- Immunoglobulin G binding protein G [Precursor] antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab181700 has not yet been referenced specifically in any publications.