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Western blot - Protein phosphatase 1 inhibitor subunit 2 antibody (ab58149)
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Product Name
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Protein phosphatase 1 inhibitor subunit 2 antibody
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See all Protein phosphatase 1 inhibitor subunit 2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Protein phosphatase 1 inhibitor subunit 2
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Immunogen
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Recombinant fragment: MAASTASHRP IKGILKNKTS TTSSMVASAE QPRGNVDEEL SKKSQKWDEM NILATYHPAD KDYGLMKIDE PSTPYHSMMG DDEDACSDTE ATEAMAPDIL , corresponding to amino acids 1-101 of Human Protein phosphatase 1 inhibitor subunit 2
BLAST 'MAASTASHRP IKGILKNKTS TTSSMVASAE QPRGNVDEEL SKKSQKWDEM NILATYHPAD KDYGLMKIDE PSTPYHSMMG DDEDACSDTE ATEAMAPDIL ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
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Relevance
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Inhibitor-2 (I-2) exists as a heterodimer with protein phosphatase type-1 (PP1), termed MgATP-dependent phosphatase. I-2 binds and forms an inactive complex with PP1 in its unphosphorylated state. This complex is activated through a series of phosphorylations on serine and threonine residues in I-2 that increases phosphatase activity. Multiple kinases have been implicated in phosphorylation of I-2 at threonine 72, namely GSK-3, cdc2 and ERK1. Casein kinase II phosphorylates I-2 at serine residues, which in turn enhances threonine phosphorylation by GSK-3.
Recent evidence has shown that phosphorylation at threonine 72 peaks during prophase of the cell cycle and is localized in the centrosomes. The I-2/PP1 complex also binds neurabin and the kinases Nek2, KPI-2, and Aurora-A. Regulation of I-2/PP1 has been shown to be important in cell cycle, gene expression, ion gating, and neuromodulation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Protein phosphatase 1 inhibitor subunit 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Protein phosphatase 1 inhibitor subunit 2 antibody images:
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 Western blot - Protein phosphatase 1 inhibitor subunit 2 antibody (ab58149)
Western blot against tagged recombinant protein immunogen using ab58149 Protein phosphatase 1 inhibitor subunit 2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
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Search PubMed (MEDLINE) for references to Protein phosphatase 1 inhibitor subunit 2
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