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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
Overview
Product name
Anti-Protein phosphatase 1 inhibitor subunit 2 antibody
See all Protein phosphatase 1 inhibitor subunit 2 products (6) ...
Description
Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2
Specificity
ab73948 detects endogenous levels of total Protein phosphatase 1 inhibitor subunit 2 protein.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic non-phosphopeptide derived from human Protein phosphatase 1 inhibitor subunit 2 around the phosphorylation site of serine 120 and serine 121 (Q-E-SP-SP-G-E).
Positive control
Human breast carcinoma tissue. Extracts from Jurkat cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
Inhibitor of protein-phosphatase 1.
Sequence similarities
Belongs to the protein phosphatase inhibitor 2 family.
Post-translational
modifications
Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
Target information above from: UniProt accessionP41236
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- IPP 2 antibody
- IPP-2 antibody
- IPP2 antibody
see all
Database links
- Entrez Gene: 5504 Human
- Entrez Gene: 66849 Mouse
- Entrez Gene: 192361 Rat
- Omim: 601792 Human
- SwissProt: P41236 Human
- SwissProt: Q9DCL8 Mouse
- SwissProt: P50411 Rat
- Unigene: 535731 Human
see all
Anti-Protein phosphatase 1 inhibitor subunit 2 antibody images:
Western blot - Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948)
All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 23 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab73948, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
References for Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948)
ab73948 has not yet been referenced specifically in any publications.
Publishing research using ab73948? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"