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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
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Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab27850? Please let us know so that we can cite the reference in this datasheet
ab27850 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry - Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
Western blot - Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution
Lane 1 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with NO added peptide
Lane 2 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with non-phosphopeptide corresponding to the immunogen
Lane 3 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with generic phosphothreonine containing peptide
Lane 4 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with phosphopeptide immunogen
Secondary
F(ab')2 ant-rabbit IgG HRP conjugate
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 44 kDa (why is the actual band size different from the predicted?)
Western blot - Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution
Lane 1 : HeLa cell extract with NO nocodazole
Lane 2 : HeLa cell extract with 200ng/ml nocodazole for 16 hours
Lane 3 : RAW 264.7 cell extract with NO nocodazole
Lane 4 : RAW 264.7 cell extract with 200ng/ml nocodazole for 16 hours
Lane 5 : HeLa cell extract with an antibody recognizing total human IPP2 protein
Lane 6 : HeLa cell extract with an antibody recognizing total human IPP2 protein
Secondary
F(ab')2 ant-rabbit IgG HRP conjugate
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 44 kDa (why is the actual band size different from the predicted?)
Additional bands at : 44/46 kDa (possible dimer),46 kDa,75 kDa. We are unsure as to the identity of these extra bands.
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