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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
Overview
Product name
Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody
See all Protein phosphatase 1 inhibitor subunit 2 products (6) ...
Description
Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2 (phospho T72)
Specificity
ab27850 recognises Inhibitor-2.
Tested applications
WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide (Human) - derived from the region that contains threonine 72.
Positive control
HeLa or RAW 264.7 cells treated with nocodazole [200ng/ml] for 16 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, 1.0 mg/mL BSA (IgG, protease free), Dulbecco's PBS [without magnesium and calcium ions]. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Inhibitor-2. The final product is generated by affinity chromatography using an Inhibitor-2 -derived peptide that is phosphorylated at threonine 72.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
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Immunocytochemistry - Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
(enlarge)
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Western blot - Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
(enlarge)
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Western blot - Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 23 kDa.
ICC: 1/100
Target
Function
Inhibitor of protein-phosphatase 1.
Sequence similarities
Belongs to the protein phosphatase inhibitor 2 family.
Post-translational
modifications
Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
Target information above from: UniProt accessionP41236
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- IPP 2 antibody
- IPP-2 antibody
- IPP2 antibody
see all
Database links
- Entrez Gene: 5504 Human
- Entrez Gene: 66849 Mouse
- Omim: 601792 Human
- SwissProt: P41236 Human
- SwissProt: Q9DCL8 Mouse
- Unigene: 535731 Human
- Unigene: 291593 Mouse
Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody images:
Western blot - Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution
Lane 1 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with NO added peptide
Lane 2 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with non-phosphopeptide corresponding to the immunogen
Lane 3 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with generic phosphothreonine containing peptide
Lane 4 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with phosphopeptide immunogen
Secondary
F(ab')2 ant-rabbit IgG HRP conjugate
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 44 kDa (why is the actual band size different from the predicted?)
Western blot - Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution
Lane 1 : HeLa cell extract with NO nocodazole
Lane 2 : HeLa cell extract with 200ng/ml nocodazole for 16 hours
Lane 3 : RAW 264.7 cell extract with NO nocodazole
Lane 4 : RAW 264.7 cell extract with 200ng/ml nocodazole for 16 hours
Lane 5 : HeLa cell extract with an antibody recognizing total human IPP2 protein
Lane 6 : HeLa cell extract with an antibody recognizing total human IPP2 protein
Secondary
F(ab')2 ant-rabbit IgG HRP conjugate
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 44 kDa (why is the actual band size different from the predicted?)
Additional bands at : 44/46 kDa (possible dimer),46 kDa,75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
ab27850 has not yet been referenced specifically in any publications.
Publishing research using ab27850? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"