Products:Signal Transduction >> Metabolism >> Energy Metabolism
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In the description section it is specified that the Ab is raised against a synthetic peptide corresponding to residues in the N-terminus of Hu I-1. Can you specify which residues are these (ex: aa 41-55)? |
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ANSWER: |
The peptide used is derived from the general region of 1-25 aa at the N-terminus. |
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I am interested in purchasing I-1 antibody and was wondering if this antibody cross reacts with DARPP-32. thanks in advance. |
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ANSWER: |
Thank you for your enquiry. We have never directly tested this, but according to sequence alignment, ab40877 will not cross react with darpp32. If you have any other questions, please do not hesitate to contact me. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Protein phosphotase inhibitor 1 antibody [EP902Y] (ab40877) at 1/40000 dilution + Rat brain at 10 µg
Predicted band size : 19 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of paraffin-embedded human normal brain tissue using ab40877 diluted 1:100.
ICC/IF image of ab40877 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40877, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing SH-SY5Y cells stained with ab40877 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40877, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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