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Product Name
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Proteinase K protein (Active)
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Product type
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Proteins and Peptides
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Peptide
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Full length native protein isolated from Tritirachium album.
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Tested applications
(see key)
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ChIP, ISH, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution.
ISH: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
This enzyme can be used with studies using ab52579, ab46540 and ab46666.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP Related Products >> ChIP Related Products
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
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Relevance
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Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name.
Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.
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Storage buffer
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Preservative: None
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Form
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Lyophilised
Reconstitute with 1ml of 50mM Tris/HCI buffer (pH7.6).
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Warning: This product is irritating to eyes, respiratory system and skin, and may cause sensitization by inhalation.
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