Overview
Product nameProteinase K protein (Active)
Protein descriptionFull length native protein isolated from Tritirachium album.
Expression hostNative
Properties
FormLyophilised:Reconstitute with 1ml of 50mM Tris/HCI buffer (pH7.6).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Concentration information loading...
Additional notesWarning: This product is irritating to eyes, respiratory system and skin, and may cause sensitization by inhalation.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab51501
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| In Situ Hybridisation |
|
| ChIP |
|
| SDS-PAGE |
|
Application notesChIP: Use at an assay dependent dilution.
ISH: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
This enzyme can be used with studies using ab52579, ab46540 and ab46666.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
Endopeptidase KTritirachium alkaline proteinase
RelevanceProteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name.
Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.
References for Proteinase K protein (Active) (ab51501)
ab51501
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
