![Western blot - Anti-Psoriasin antibody [47C1068] (ab13680) image](#abcam1-13680.jpg)
 image](#Psoriasin-Primary-antibodies-ab13680-1.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-Psoriasin antibody [47C1068]
See all Psoriasin products (7) ...
Description
Mouse monoclonal [47C1068] to Psoriasin
Tested applications
IP, ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Positive control
Confluent MCF10A cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is affinity purified.
Clonality
Monoclonal
Clone number
47C1068
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Tumor biomarkers >> Other
Cancer >> Signal transduction >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 5 µg/ml
Flow Cyt: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 11 kDa (predicted molecular weight: 12.1 kDa).
IHC-Fr: Use at an assay dependent dilution. ((PMID 18606705).)
IHC-P: Use at an assay dependent dilution.
Target
Tissue specificity
Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients.
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains.
Cellular localization
Cytoplasm. Secreted. Secreted by a non-classical secretory pathway.
Target information above from: UniProt accessionP31151
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HID 5 antibody
- Protein S100 A7 antibody
- Protein S100-A7 antibody
see all
Database links
- Entrez Gene: 6278 Human
- Omim: 600353 Human
- SwissProt: P31151 Human
- SwissProt: Q5SY67 Human
- SwissProt: Q6FGE3 Human
- Unigene: 112408 Human
Anti-Psoriasin antibody [47C1068] images:
Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)
![Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)](/ps/datasheet/Images/13/ab13680/abcam1-13680.jpg)
Predicted band size : 12.1 kDa
Western blot analysis of Psoriasin in confluent MCF10A cells using ab13680 at 1 µg/ml.
Immunocytochemistry/ Immunofluorescence-Psoriasin antibody [47C1068](ab13680)
](/ps/datasheet/images/13/ab13680/Psoriasin-Primary-antibodies-ab13680-1.jpg)
ICC/IF image of ab13680 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13680, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Psoriasin antibody [47C1068] (ab13680)
This product has been referenced in:
- West NR & Watson PH S100A7 (psoriasin) is induced by the proinflammatory cytokines oncostatin-M and interleukin-6 in human breast cancer. Oncogene : (2010). WB; Human.Read more (PubMed: 20101226) »
- Wolf Ret al. Highly homologous hS100A15 and hS100A7 proteins are distinctly expressed in normal breast tissue and breast cancer. Cancer Lett 277:101-7 (2009). WB, IHC-Fr; Human.Read more (PubMed: 19136201) »
See all 7 publications for this product
Publishing research using ab13680? Please let us know so that we can cite the reference in this datasheet
![Psoriasin antibody [47C1068] for Flow Cytometry in Human (13680)](/ps/datasheet/images/13/ab13680/Psoriasin-Primary-antibodies-ab13680-3.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"