Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-Pumilio 2 antibody
See all Pumilio 2 products (6) ...
Description
Goat polyclonal to Pumilio 2
Specificity
ab32894 recognises PUM2.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee
Immunogen
Synthetic peptide: C-DQNRDLKQGDDDDSK, corresponding to Internal sequence amino acids 141-155 of Human Pumilio 2
C-DQNRDLKQ GDDDDSK
Positive control
Human Epstein-Barr virus immortilised lymphoblastoid lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
-
Western blot - Pumilio 2 antibody (ab32894)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/4000ELISA: 1/4000
WB: Use a concentration of 1 - 3 µg/ml.Detects a band of approximately 110 kDa (predicted molecular weight: 114 kDa).
Target
Relevance
Pumilio 2, a human homolog of Pumilio, a protein required to maintain germ line stem cells in Drosophila and Caenorhabditis elegans, forms a stable complex with DAZ through the same functional domain required for RNA binding, protein-protein interactions and rescue of Pumilio mutations in flies. Pumilio 2 is expressed predominantly in human embryonic stem cells and germ cells and colocalizes with DAZ and DAZL in germ cells. Pumilio 2 is a component of conserved cellular machinery that may be required for germ cell development.
Cellular localization
Cytoplasmic
Alternative names
- FLJ36528 antibody
- KIAA0235 antibody
- KIAA0235 antibody
see all
Database links
- Entrez Gene: 23369 Human
- SwissProt: Q8TB72 Human
- Unigene: 467824 Human
Anti-Pumilio 2 antibody images:
Western blot - Pumilio 2 antibody (ab32894)
All lanes : Anti-Pumilio 2 antibody (ab32894) at 1 µg/ml
Lane 1 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - without blocking with the immunizing peptide
Lane 2 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - blocking with the immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size : 114 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,65 kDa,75 kDa. We are unsure as to the identity of these extra bands.
NB: Some minor bands are resistant to blocking by the immunizing peptide.
References for Anti-Pumilio 2 antibody (ab32894)
ab32894 has not yet been referenced specifically in any publications.
Publishing research using ab32894? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"