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Overview

  • Product namePyruvate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 200 µM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Pyruvate Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured. The kit detects 1-200 µM pyruvate.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Pyruvate is a central molecule in metabolism through which sugars enter the citric acid cycle. Pyruvate can be converted to carbohydrates during gluconeogenesis or to fatty acids via acetyl CoA. High levels of pyruvate are associated with liver disease and genetic disorders. Pyruvate has also been used to stimulate metabolism leading to loss of body weight.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevancePyruvate is a key intersection molecule in the netwrok of metabolic pathways. Pyruvate can be made from glucose through glycolysism converted back to carbohydrates via gluconeogenesis, or to fatty acids or energy via acetyl CoA. It can also be used to construct the amino acid alanine and be converted to ethanol. Glucose is converted to pyruvate which is then converted into acetyl CoA, the main input for the citric acid cycle (TCA). If insufficent oxygen is available, pyruvate breaks down anaerobically, creating lactate in animals and ethanol in plants and microorganisms.

    • Applications

    Our Abpromise guarantee covers the use of ab65342 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Pyruvate Assay Kit images

    • Functional Studies - Pyruvate Assay Kit (ab65342)
      Functional Studies - Pyruvate Assay Kit (ab65342)
      Standard calibration curve of Pyruvate.

    Protocols

    References for Pyruvate Assay Kit (ab65342)

    ab65342 has not yet been referenced specifically in any publications.

    Publishing research using ab65342 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65342 Submit a Question for ab65342

    Displaying 1 - 10 of 22 results for Abreviews and Q&A

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    Customer kindly contacted us inquiring use of this product in yeast and e.coli samples.

    I have spoken with the lab regarding the use of this kit in yeast and bacteria. They agree that while we have optimized this assay protocol for samples of mammalian origin, theoretically this assay should work with samples from multiple origins including ...

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    please i want to know sample appropriate for the assay is it whole blood or serum or what? and do i have to do some preparations for the sample before the assay?


    You can use plasma samples with this assay. Optimal dilutions have to be determined using a pilot experiment with different volumes.

    We are using human and mouse urine that has been filtered to remove protein (as suggested by the kit insert) and then frozen once at -70° before assaying. It appears there is some analyte in the urine that inhibits the assay. We have assayed plasma and ki...

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    Uric acid in urine is going to strongly interfere with the assay so besides doing the normal standard curve, the lab recommends to also do standard curve in the presence of X volume of diluted urine (may be 4-15 ul depends). Due to the presence of uric ac...

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    Customer kindly contacted us as the pyruvate assay buffer when thawed had flake precipitate out. Should this be of concern? What is the recommended way to resuspend these? Would heating to 37C adversely affect the buffer?

    The lab has advised me that you can keep the buffer at 37°C for some time to see if it helps. The 37°C for a short time should not adversely affect the assay; in fact many clients do keep the buffer bottle at this temp water bath while thawing it ...

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    A. General Information
    - Catalogue number: ab83429; ab65342
    - Lot number:
    - Purchase order number/ order date or preferably the Abcam order number:
    - The kits were stored at -20C.
    B. Description of the problem
    - Are the signal...

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    Thank you for taking the time to complete our questionnaire and providing this further information. I am sorry to hear you have had difficulty obtaining satisfactory results from our D-Lactate Assay Kit (Colorimetric) (ab83429) and our Pyruvate Assay Kit ...

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    Thanks for your reply. I would also like to know if there is a guideline for the period time that the cells need to be lysed in the assay buffer for complete lysis to occur.

    Regards,

    Thank you for contacting us.

    Start with ˜2X106 cells, suspend the cell pellet 500 μl (or ˜4 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the ce...

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    What is the minimum amount of assay buffer that could be used to prepare lysates?

    Thank you for your email.

    We recommend using this kit with 2x106 cells per 500ul of assay buffer. This cell number however is for guidelines only; the number should be optimized as it varies based on cell line type.

    One way to prepare ce...

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    Hi
    I bought your Pyruvate assay kit to check the Pyruvate level in cells. I am missing instructions how to treat cells to be able to assay the pyruvate content as well as an idear about the cell number needed. Is your assay buffer an Lysis buffer or ...

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    Thank you for your enquiry regarding ab65342.

    I apologize for my delayed response but was out of the office yesterday.

    You can homogenize about 2 x 10^6 cells with 500 µl of the assay buffer, centrifuge it and take the supernatan...

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    Did you have a pyruvate test kit available for human plasma? Patients with metabolic disorders.

    The assay kit ab65342 is capable of detecting pyruvate in human plasma. Please note, our products are for "Research Use Only" (RUO), and are not intended for diagnostic use. Also note that due to the presence of LDH in serum and plasma, care must be taken...

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    Please find the attached file and help me out.







    cat no. ab56342 and lot no. GR77215-2.









    Diluted the pyruvate standard to 1 nmol/microlit by adding 10 m...

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    Thank you for your message and for providing this further information.


    I appreciate the time your customer hasspent on these experiments, and would like to offer a free of charge replacementor credit note in compensation (providing the pro...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"