Products:Signal Transduction >> Metabolism >> Energy Metabolism
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ab67592 has been referenced in 3 publications.
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Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 43 kDa
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution
Lane 1 : Pyruvate Dehydrogenase E1-alpha subunit transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of formalin fixed and paraffin embedded human stomach tissue labelled with ab67592 at 1.5µg/ml.
ICC/IF image of ab67592 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67592, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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