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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody
See all Pyruvate Dehydrogenase E1-alpha subunit products (8) ...
Description
Mouse polyclonal to Pyruvate Dehydrogenase E1-alpha subunit
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Full length PDHA1 protein (Human)
Positive control
Human liver tissue lysate, PDHA1 transfected lysate and Human stomach tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
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Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P: Use a concentration of 1.5 µg/mlPerform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP08559
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MSP03 antibody
- MSP07 antibody
- ODPA_HUMAN antibody
see all
Database links
- Entrez Gene: 5160 Human
- Entrez Gene: 18597 Mouse
- Omim: 300502 Human
- SwissProt: P08559 Human
- SwissProt: P35486 Mouse
- Unigene: 530331 Human
- Unigene: 34775 Mouse
Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody images:
Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 43 kDa
Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution
Lane 1 : Pyruvate Dehydrogenase E1-alpha subunit transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
Immunohistochemical analysis of formalin fixed and paraffin embedded human stomach tissue labelled with ab67592 at 1.5µg/ml.
Immunocytochemistry/ Immunofluorescence-Pyruvate Dehydrogenase E1-alpha subunit antibody(ab67592)
ICC/IF image of ab67592 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67592, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
This product has been referenced in:
- Wolf Aet al. Hexokinase 2 is a key mediator of aerobic glycolysis and promotes tumor growth in human glioblastoma multiforme. J Exp Med 208:313-26 (2011). WB; Human.Read more (PubMed: 21242296) »
- Arastu-Kapur Set al. Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a link to clinical adverse events. Clin Cancer Res 17:2734-43 (2011). WB; Human.Read more (PubMed: 21364033) »
See all 3 publications for this product
Publishing research using ab67592? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"