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Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
(enlarge)
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Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
(enlarge)
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Product Name
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Pyruvate Dehydrogenase E1-alpha subunit antibody
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See all Pyruvate Dehydrogenase E1-alpha subunit antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to Pyruvate Dehydrogenase E1-alpha subunit
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Immunogen
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Full length PDHA1 protein (Human)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver tissue lysate, PDHA1 transfected lysate and Human stomach tissue
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Cellular localization
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Mitochondrial
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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PDH1 E1 alpha is the alpha subunit of pyruvate decarboxylase, the first of 3 enzymes in the pyruvate dehydrogenase (PDH) complex. The beta subunit of the E1 enzyme is encoded by the PDHB gene.
The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: PDH1 E1; dihydrolipoyl transacetylase E2C; and dihydrolipoyl dehydrogenase E3. The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Pyruvate Dehydrogenase E1-alpha subunit antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Pyruvate Dehydrogenase E1-alpha subunit antibody images, references, products related to ab67592 and other tools.
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Pyruvate Dehydrogenase E1-alpha subunit antibody images:
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 Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 43 kDa
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Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
All lanes : Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution
Lane 1 : Pyruvate Dehydrogenase E1-alpha subunit transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
Immunohistochemical analysis of formalin fixed and paraffin embedded human stomach tissue labelled with ab67592 at 1.5µg/ml.
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References for Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
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This product has been used in:
Boudina S et al. Contribution of impaired myocardial insulin signaling to mitochondrial dysfunction and oxidative stress in the heart. Circulation 119:1272-83 (2009). WB; Mouse. PubMed: 19237663
If you publish research using ab67592 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Pyruvate Dehydrogenase E1-alpha subunit
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Pyruvate Dehydrogenase E1-alpha subunit antibody - more information
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Customer reviews (feedback) regarding Pyruvate Dehydrogenase E1-alpha subunit antibody |
Customer FAQs regarding Pyruvate Dehydrogenase E1-alpha subunit antibody |
Protocols for Pyruvate Dehydrogenase E1-alpha subunit antibody |
Price and availability of products related to Pyruvate Dehydrogenase E1-alpha subunit antibody |
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Pyruvate Dehydrogenase E1-alpha subunit antibody - related products:
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Pyruvate Dehydrogenase E1-alpha subunit antibody - other tools:
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Contact Abcam with a Technical Enquiry about Pyruvate Dehydrogenase E1-alpha subunit antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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